We narrowed to 10,965 results for: T7

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pcDNA3.1_SNORD2 probe

Plasmid

#73070

Purpose

antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD2 (SNORD2 Human)

Expression

Mammalian

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SNORD60 probe

Plasmid

#73071

Purpose

antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD60 (SNORD60 Human)

Expression

Mammalian

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Tags

6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-C-SH2+IA

Plasmid

#111418

Purpose

express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

Insert

murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-N-SH2+IA

Plasmid

#111273

Purpose

express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)

Tags

HHHHHHENLYFQG

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-HypD

Plasmid

#110288

Purpose

Expresses t-4-hydroxy-L-proline dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

t-4-Hydroxy-L-proline Dehydratase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-H166Q

Plasmid

#110179

Purpose

Expresses propanediol dehydratase mutant H166Q in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

H166Q mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-E440Q

Plasmid

#110178

Purpose

Expresses propanediol dehydratase mutant E440Q in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

E440Q mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-C438S

Plasmid

#110177

Purpose

Expresses propanediol dehydratase mutant C438S in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

C438S mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-G817A

Plasmid

#110174

Purpose

Expresses propanediol dehydratase mutant G817A in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

G817A mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Plasmid

#101207

Purpose

Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A and Q695A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

Plasmid

#101201

Purpose

Bacterial expression plasmid for SpCas9, REC3 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C and S960C

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

Plasmid

#101214

Purpose

Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S355C/S867C/K855A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S355C, S867C and K855A

Promoter

T7

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

Plasmid

#101200

Purpose

Bacterial expression plasmid for SpCas9, REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C and D273C

Promoter

T7

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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