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GGE021

Plasmid

#120778

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position2-3. When using the GG platform for expressing only two gene (T2-3_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2-3_Lip2

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE002

Plasmid

#120739

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_GAPdh

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE015

Plasmid

#120770

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2_Lip2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE177

Plasmid

#120766

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Xpr2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGD165

Plasmid

#132370

Purpose

contains the sequence of eGFP for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-eGFP

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD250

Plasmid

#132371

Purpose

contains the sequence of mClover3 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-mClover3

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD251

Plasmid

#132372

Purpose

contains the sequence of mCerulean3 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-mCerulean3

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD255

Plasmid

#132376

Purpose

contains the sequence of Venus for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-Venus

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTK565

Plasmid

#114690

Purpose

SNAP-tagging at DHC's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK586

Plasmid

#114716

Purpose

SNAP-tagging at LGN's N-terminal locus (BSD)

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pANY3

Plasmid

#112197

Purpose

This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.

Depositor

Yingfeng An

Article

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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TP901 GFP BAC Reporter

Plasmid

#78216

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
pCri-8a

Plasmid

#61317

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only
pCri-7b

Plasmid

#61330

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pFUS_A4B

Plasmid

#43854

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pJL1-FETCH1

Plasmid

#210644

Purpose

In vitro expression of FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-X4

Plasmid

#176154

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3

Plasmid

#171497

Purpose

T7 promotor drives in vitro transcription of mClover3 mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

mClover3

Use

Pgehme

Promoter

T7

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETA_crE6

Plasmid

#172928

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

Depositor

Arnold Boersma

Article

Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

Insert

CrowdingSensorE6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MTK6_016

Plasmid

#124058

Purpose

Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

WPRE (extended)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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