We narrowed to 19,334 results for: Kin

1. pHAGE-EF1aL-TagBFP-W

   Plasmid

   #126687

   Purpose

   Constitutive, ubiquitous blue fluorescence protein (BFP) expression.

   Depositor

   Darrell Kotton

   Article

   Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

   Insert

   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1aL

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-Ufm1 ∆C3

   Plasmid

   #133866

   Purpose

   Expression of human UFM1 mutant lacking the last three C-terminal residues

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV-PGKpuro2ABFP

   Plasmid

   #122372

   Purpose

   Expresses puro-2A-BFP in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   Puro-2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET51b-His-TEV-CLIP-tag

   Plasmid

   #167274

   Purpose

   Overexpression of CLIP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   CLIP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiV_Neo_LKB1

   Plasmid

   #108111

   Purpose

   Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   LKB1 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFPC1-Cep83

   Plasmid

   #128874

   Purpose

   For mammalian expression of mouse Cep83

   Depositor

   Aimin Liu

   Article

   Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

   Insert

   Cep83 (Ccdc41 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3 NSE promoter

   Plasmid

   #11606

   Depositor

   Rosalyn Adam

   Article

   Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

   Insert

   NSE promoter (ENO2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ExRai-AMPKAR(T/A)

   Plasmid

   #192447

   Purpose

   Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR(T/A)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Thr 6 in AMPK substrate mutated to Ala.

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMP1237

   Plasmid

   #119262

   Purpose

   for cloning new sgRNAs

   Depositor

   Jason Peters, Carol Gross, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-MBP-2xNLS-tdTomato

    Plasmid

    #104054

    Purpose

    An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-tdTomato
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    MBP

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-AF8

    Plasmid

    #117803

    Purpose

    mammalian expression of GFP-tagged human centromere protein A

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2000 Aug;25(4):253-61

    Insert

    CENP-A (CENPA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBa.PEX3-tdTomato-FKBP

    Plasmid

    #193941

    Purpose

    Mammalian expression of tdTomato-tagged PEX3

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    PEX3 (PEX3 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-4T-1-3xFLAG-HSP27

    Plasmid

    #129229

    Purpose

    Encodes FLAG tagged human HSP27 for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    HSP27 (HSPB1 Human)
    

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PWPXLD-GFP-Numb-WT

    Plasmid

    #133751

    Purpose

    Wild-type Numb tagged with GFP cloned into PWPXLD

    Depositor

    Chay Kuo

    Article

    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

    Insert

    GFP-Numb (Numb Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1105-Desmoglein2-FLAG-eGFP

    Plasmid

    #36989

    Depositor

    Kathleen Green

    Article

    Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Ep

    Insert

    Dsg2 (DSG2 Human)
    

    Use

    Tags

    FLAG and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-N-TEV-Twin-Strep

    Plasmid

    #202526

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site and Twin-strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-GFP (F64L, S65T, V163A)-TRP1

    Plasmid

    #53184

    Purpose

    chromosomal tagging with stable GFP

    Depositor

    John Pringle

    Article

    Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP (F64L, S65T, V163A)

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA4-Vps34-Flag

    Plasmid

    #24398

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Vps34 (PIK3C3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. AD59_pEP.DonorCLYBL.TS

    Plasmid

    #199227

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFloat.cspA_v1

    Plasmid

    #133071

    Purpose

    Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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