We narrowed to 18,937 results for: Tec

1. pU6-(BbsI)_CBh-Cas9-T2A-BFP

   Plasmid

   #64323

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-EBFP2

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. MT-AvrXa10-FT

   Plasmid

   #234373

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

   Depositor

   Michael Smanski

   Article

   Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

   Inserts

   NbGP41-GFP-AvrXa10-GB1
   dCas9-24XGP41
   gRNAs targeting FT locus
   RFP
   

   Use

   Synthetic Biology

   Tags

   GB1, GFP, and GP41

   Expression

   Plant

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACBE-Q

   Plasmid

   #204608

   Purpose

   Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1 flag-cFLIP

   Plasmid

   #211529

   Purpose

   Expresses flag-tagged cFLIP (CFLAR)

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   cFLIP (CFLAR Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL-mouse cGAS #1-gRNA-Cas9-Puro

   Plasmid

   #186890

   Purpose

   gRNA targeting mouse cGAS (with Cas9 insert)

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Cas9 (Cgas Mouse)
   

   Use

   Lentiviral

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-UBC-XRI-HA

   Plasmid

   #178056

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-HA
   

   Use

   AAV

   Tags

   HA-MBP

   Expression

   Mammalian

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT305 Pa01 Lenti pEF-attB LSR GFP

   Plasmid

   #184948

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01 Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKL038 Lenti U6 dCas12a gRNA Puro mCherry

   Plasmid

   #195546

   Purpose

   dCas12a gRNA expression backbone

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti U6- empty cassette_Direct repeat_puro_mcherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CARPID BASU-dCasRx

    Plasmid

    #153209

    Purpose

    express CARPID BASU-dCasRx fusion protein in mammalian cells

    Depositor

    Jian Yan, Liang Zhang

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    BASU-dCasRx
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mARGAna (cassette 1, transient)

    Plasmid

    #197588

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid

    #190112

    Purpose

    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Inserts

    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    

    Use

    AAV

    Tags

    bpNLS and bpNLS-P2A-eGFP

    Mutation

    mutations from RT in PE2 and truncation of RNAse …

    Promoter

    EFS and U6, H1

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT301 BxBi Lenti pEF-attB LSR GFP

    Plasmid

    #184944

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    BxBi Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT306 Kp03 Lenti pEF-attB LSR GFP

    Plasmid

    #184949

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJR89

    Plasmid

    #140096

    Purpose

    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT302 Ec03 Lenti pEF-attB LSR GFP

    Plasmid

    #184945

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT303 Ec04 Lenti pEF-attB LSR GFP

    Plasmid

    #184946

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec04 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT304 Ec07 Lenti pEF-attB LSR GFP

    Plasmid

    #184947

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec07 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pscALPSpuro-MDA5-S88D

    Plasmid

    #174226

    Purpose

    Expresses human MDA5 S88D mutant

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MDA5 (IFIH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S88D

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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