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pAG79-attL1-pegRNA-attL2

Plasmid

#213050

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TrxRFP1

Plasmid

#98996

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-mito-Citron1

Plasmid

#134305

Purpose

mitochondria-targeted direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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MaLionR

Plasmid

#113908

Purpose

Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionR

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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mCRISPRed pRSETB

Plasmid

#159454

Purpose

Expresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRed

Depositor

Oliver Griesbeck

Article

Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

Insert

mCRISPRed

Tags

10 x His-Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCR8-attL3-pegRNA-attL2

Plasmid

#213052

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL1-pegRNA-attR3

Plasmid

#213051

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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MscL(G22S)-c-term-cpGFP-ERexp

Plasmid

#195346

Purpose

Control plasmid for the MscL tension biosensor

Depositor

Allen Liu

Article

Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

Insert

MscL(G22S)-c-term-cpGFP-ERexp

Use

Lentiviral

Expression

Mammalian

Promoter

CMVd2

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-8XHis-mCherry-Omp25

Plasmid

#236399

Purpose

Elutable MitoTag-AP system

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

8XHis-mCherry-Omp25

Use

Retroviral

Tags

8XHis-tag and mCherry

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MaLionG

Plasmid

#113906

Purpose

Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Pol

Plasmid

#178782

Purpose

Inducible expression of Taq DNA polymerase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Polymerase

Expression

Bacterial

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis T4 DNA Ligase

Plasmid

#206207

Purpose

Inducible expression of T4 DNA ligase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

T4 DNA ligase

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-mE1

Plasmid

#32534

Depositor

Jorge Eduardo Azevedo

Article

Carvalho et al Mol Biotechnol. 2011 Oct 20.

Insert

E1, ubiquitin-activating enzyme (Uba1 Mouse)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

Plasmid

#60906

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 WT in pcDNA3.1

Plasmid

#118213

Purpose

Expresses HA-UBE2E1 in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

none

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Expression

Bacterial and Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA Scramble

Plasmid

#162011

Purpose

Lentiviral negative control vector containing scramble shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

scramble shRNA

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCap-SG5-Apr

Plasmid

#227579

Purpose

Capture plasmid with apramycin resistance and pSG5 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Type

Empty backbone

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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p3258 pCMV hUBC9 wt HA

Plasmid

#14438

Depositor

Peter Howley

Article

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Insert

UBC9 (UBE2I Human)

Tags

HA

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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MaLionB

Plasmid

#113910

Purpose

Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionB

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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