We narrowed to 9,895 results for: EPO
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Plasmid#97311PurposeMMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb MMEJ donor
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterU6, EF1aAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TUBG1
Plasmid#171967PurposepGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion proteinDepositorInsertTUBG1 (TUBG1 Human)
UseTagsGSTExpressionBacterialMutationR275H (Please see depositor comments)PromoterAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-331-3p
Plasmid#103444PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-331-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-331-3p target (MIR331 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo 1-159
Plasmid#186016Purpose2xHis-LRRK2 Armadillo 1-159DepositorInsert2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)
UseTagsExpressionBacterialMutationSee depositor's comments below.PromoterAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS2274
Plasmid#111900PurposessAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry
Plasmid#179435PurposeDual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-coreHS4-mch (SC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro2 Δ593-618
Plasmid#47901PurposeExpresses myc tagged Miro2 lacking transmembrane domainDepositorInsertMiro2 Δ593-618 (RHOT2 Human)
UseTagsmycExpressionMammalianMutationΔ593-618, lacks transmembrane domain. Also see De…PromoterCMVAvailable SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-ACTB_sgRNA
Plasmid#183884PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.DepositorAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-485-3p
Plasmid#103553PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-485-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-485-3p target (MIR485 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-TGFB2
Plasmid#185556PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting TGFB2DepositorInsertTGFB2 gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-148a-3p
Plasmid#103250PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-148a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-148a-3p target (MIR148A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irG4kwh
Plasmid#44723DepositorInsertspCMV-D2i promoter
EGFP
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-MFAP2
Plasmid#185555PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting MFAP2DepositorInsertMFAP2 gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-SOX4
Plasmid#185552PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting SOX4DepositorInsertSOX4 gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
human SLC12A5-V5-HA-2A-Luciferase repair template
Plasmid#158576PurposeFor targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInserthuman SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
UseTagsV5 and HA on the endogenous KCC2 protein productExpressionMammalianMutationPromoterAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIS1 ATP2B1
Plasmid#60788PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and wild-type miR-155 sitesDepositorInsertATP2B1 3'UTR and wild-type miR-155 binding site (ATP2B1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterthymidine kinase (HSV-TK promoter)Available SinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-449a
Plasmid#103528PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-449a target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-449a target (MIR449A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97310PurposeNHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb NHEJ donor
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterU6, EF1aAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-522-3p
Plasmid#103614PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-522-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-522-3p target (MIR522 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS1097
Plasmid#29028PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle201 (SLC6A5 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable SinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only