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pNIC-HIS6-GST-TEV-GG

Plasmid

#215801

Purpose

Cloning of target gene with N terminal His6 GST tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Cav-RA

Plasmid

#209881

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

CAVIN1

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-HA-eLACCO2.1-NGR

Plasmid

#207989

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yuki Kambe

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

AAV

Promoter

CaMKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKI1

Plasmid

#199118

Purpose

Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

Depositor

Jeff Gelles

Article

Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

Insert

H6-SNAP-RapA

Tags

6xHis and SNAP-tag

Expression

Bacterial

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb713

Plasmid

#197207

Purpose

Expresses the protein of mRhubarb713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb713

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP680

Plasmid

#197203

Purpose

Expresses the protein of miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mCardinal

Plasmid

#197195

Purpose

Expresses the protein of mCardinal in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBT005

Plasmid

#177323

Purpose

MCP-SoxS (R93A) expression

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Insert

SoxS

Tags

MCP (MS2 Coat Protein)

Mutation

R93A

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-BRIC

Plasmid

#172343

Purpose

Bioluminescent Red Indicator for Calcium (bacterial expression)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BRIC

Expression

Bacterial

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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cyterm-ymScarletI pDRF1-GW

Plasmid

#168082

Purpose

Expresses Cyterm-ymScarletI to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymScarletI

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1324

Plasmid

#139874

Purpose

Expression plasmid for WT LetB (without TM region) for cryo and negative stain EM

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877)-6xHis

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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P442

Plasmid

#139728

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, promoter is ABA responsive

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_pAtRAB18:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

AtRAB18

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCO487

Plasmid

#139730

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, H3H

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_B_AtUbi10:NnH3H:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

CmYLCV

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCO488

Plasmid

#139731

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Hisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_C'_CmYLCV:NnHisps:tHsp

Use

Luciferase

Expression

Plant

Promoter

CmYLCV

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCre-AntiDsRed

Plasmid

#139674

Purpose

Cassette for testing Cre recombinase activity using a DsRed2 fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Cre recombinase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBORF-DBD

Plasmid

#127463

Purpose

ORF filtering plasmid (lambda cI associated plasmid for pAVA)

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSDMA66

Plasmid

#128355

Purpose

gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

Depositor

James Fraser

Article

Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

Insert

Noureothricin (NAT)

Use

Unspecified

Promoter

ACT1

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS15c-RFP

Plasmid

#126198

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS20k-RFP

Plasmid

#126196

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

S23200

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB13k-GFP

Plasmid

#126122

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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