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pPD864 pLP Rosa26 EF1a attP EYFP-P2A-Hygro

Plasmid

#253872

Purpose

Contains the landing pad (attP) flanked by Rosa26 homology arms to generate B16F10-LP

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

attP EYFP-P2A-Hygro

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1291 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU1

Plasmid

#253918

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1295 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU5

Plasmid

#253922

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1292 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU2

Plasmid

#253919

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1031 EF1a miRFP670 2A Blast

Plasmid

#253904

Purpose

Components for Integration Vectors: mMoClo TUPV, with EF1alpha promoter and miRFP720-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

miRFP720-P2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1294 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU4

Plasmid

#253921

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1293 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU3

Plasmid

#253920

Purpose

Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mTagBFP2-P2A-AkaLuc-2A-BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPD1402 Dox-Inducible mNeonGreen

Plasmid

#253891

Purpose

Integration Vector for mNeonGreen with the TRE3GV promoter (TUPV1), TetOn3G with the CAG promoter (TUPV2), and BlastR with the CAG promoter (TUPV3)

Depositor

Joshua Leonard

Article

Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1

Insert

mNeonGreen, TetOn3G, and BlastR

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GV, CAG, CAG

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-Emsy (mouse)

Plasmid

#236419

Purpose

transient overexpression of EMSY in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

EMSY (2210018M11Rik Mouse)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)

Plasmid

#105194

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(HC)-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)

Plasmid

#105195

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(LC)-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGMCK-0X750-luxAB-DAS+4

Plasmid

#49517

Purpose

Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

P750-luxAB-DAS+4

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Promoter

P750

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFBOH-avi-TEV-LIC_DCAF12:1-453

Plasmid

#210940

Purpose

Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Expression

Insect

Mutation

R131Q natural variant (VAR_035322)

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_EIF2A:1-427

Plasmid

#210939

Purpose

Insect expression of human EIF2A fragment M1 to T427. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF2A:M1-T427 (EIF2A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_UTP15:1-370

Plasmid

#210937

Purpose

Insect expression of human UTP15 fragment M1 to H370. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

UTP15:M1-H370 (UTP15 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TBL2:70-405

Plasmid

#210936

Purpose

Insect expression of human TBL2 fragment E70 to H405. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TBL2:E70-H405 (TBL2 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SHKBP1:182-585

Plasmid

#210935

Purpose

Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SHKBP1:T182-L585 (SHKBP1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

Q507L natural variant (VAR_036714)

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_IFT172:1-320

Plasmid

#210934

Purpose

Insect expression of human IFT172 fragment M1 to S320. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

IFT172:M1-S320 (IFT172 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR19:1-636

Plasmid

#210933

Purpose

Insect expression of human WDR19 fragment M1 to G636. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR19:M1-G636 (WDR19 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR73:1-378

Plasmid

#210932

Purpose

Insect expression of full-length human WDR73. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR73:M1-R378 (WDR73 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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