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MTK3_009

Plasmid

#123724

Purpose

Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PHYB-VP16-NLS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GWF196

Plasmid

#133613

Purpose

NS3aH1-VPR

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

NS3aH1-VPR

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-5

Plasmid

#117034

Purpose

Constitutive integrase cassette - Bxb1-Tp901

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Bxb1-Tp901

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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BB1_12_YlpGPD

Plasmid

#117820

Purpose

Promoter pGPD of Y. lipolytica for GoldenMOCS

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpGPD

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pZS2katGp-RBS33-TP901-proD-oxyR

Plasmid

#78215

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pZS1katGp-RBS31-PhiC31-proD-oxyR

Plasmid

#78217

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR

Plasmid

#87642

Purpose

For creating drosophila transgenics expressing HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Bxb1

Expression

Insect

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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HeatShock-acNPV_Bxb1_SV40UTR

Plasmid

#87641

Purpose

For creating drosophila transgenics expressing HeatShock-acNPV_Bxb1_SV40UTR

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Bxb1

Expression

Insect

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2992

Plasmid

#63642

Purpose

pCfB2992 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRAV24

Plasmid

#67716

Purpose

Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only
pCri-4b

Plasmid

#61328

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and TRX-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCri-6a

Plasmid

#61315

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

GST-tag and His-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pZSm47a luxR RBS

Plasmid

#48891

Purpose

ArsR-regulated expression of luxR

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

luxR

Use

Synthetic Biology

Expression

Bacterial

Promoter

pArsR

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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CMV-10-3xFLAG-LGP2-MIII

Plasmid

#58683

Purpose

Expresses LGP2 with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

LGP2-MIII (DHX58 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

threonine and serine of helicase motif III mutate…

Promoter

CMV

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only
pDonr201_PARG WT

Plasmid

#240315

Purpose

Entry vector for Gateway with PARG

Depositor

Junjie Chen

Article

Nie et al Elife. 2024 Apr 5;12:RP89303. doi: 10.75

Insert

PARG (PARG Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-GreenPy1Highest

Plasmid

#239049

Purpose

Bacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

GreenPy1Highest

Expression

Bacterial

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-GreenPy1Low

Plasmid

#239061

Purpose

Mammalian expression of low affinity green fluorescent pyruvate biosensor GreenPy1Low

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

GreenPy1Low

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-ApplePy1Lowest

Plasmid

#239067

Purpose

Mammalian expression of lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Lowest

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-GreenPy1Lowest

Plasmid

#239052

Purpose

Bacterial expression of lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

GreenPy1Lowest

Expression

Bacterial

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_GFP

Plasmid

#221469

Purpose

Gateway cloning of GFP overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Gateway Cloning

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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