We narrowed to 11,594 results for: EMB

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pSpyC

Plasmid

#220187

Purpose

sgRNA expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyD

Plasmid

#220188

Purpose

sgRNA expression vector - pBG28 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG28

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGM295

Plasmid

#220183

Purpose

Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

SEC61B (SEC61B Human)

Use

Lentiviral

Tags

sfGFP

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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AA371

Plasmid

#215989

Purpose

Fragmid fragment: (N' terminus) attenuate expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; u(S)ORF_v1.1; Stop; Start; HIV-NES_v1.2; HIV-NES_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_792

Plasmid

#216084

Purpose

for adding plate and well barcodes via 6-9 and 9-10 modules, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

for adding plate and well barcodes via 6-9 and 9-10 modules

Use

Lentiviral; Destination

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_131

Plasmid

#216095

Purpose

Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD97, CD4, CD26, CD274 guides

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk2a

Plasmid

#208983

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk2a (kcnk2a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-sfgfp

Plasmid

#209994

Purpose

Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

sfGFP

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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(R)pADH2(D)_P1(SBE214)

Plasmid

#195015

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(B) pADH2 (R)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_EF-act1_full

Plasmid

#202172

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 full terminator

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-act1_tr

Plasmid

#202171

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 truncated terminator

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-PT2A

Plasmid

#202138

Purpose

Level 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructs

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Dual 2A peptide

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-mito_sig_pep

Plasmid

#202134

Purpose

Level 0 plasmid containing a kozak consensus sequence and a mitochondria signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Mitochondria signal peptide

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-sec_sig_pep

Plasmid

#202133

Purpose

Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Secretion signal peptide

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-chl_sig_pep

Plasmid

#202131

Purpose

Level 0 plasmid containing a kozak consensus sequence and a chloroplast signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Chloroplast signal peptide

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pJL1-MBP-DQNAT

Plasmid

#198215

Purpose

pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

MBP_DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrSmg6_F

Plasmid

#146279

Purpose

Bacterial Expression of DrSmg6

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrSmg6 (LOC100334020 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrUPF2_F

Plasmid

#146282

Purpose

Bacterial Expression of DrUPF2

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF2 (upf2 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY

Plasmid

#193555

Purpose

Expresses mEGFP-ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

ShadowY

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pME-p3E-GLRX-roGFP2

Plasmid

#194288

Purpose

Multisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQF

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

Gateway Cloning

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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