We narrowed to 9,183 results for: Pol

1. pXPR_023d-sgCiBIRC6-5

   Plasmid

   #202448

   Purpose

   Expression of a CRISPRi guide targeting BIRC6

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   BIRC6 gRNA (BIRC6 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXPR_003-sgBIRC6-4

   Plasmid

   #202438

   Purpose

   Expression of a guide RNA targeting BIRC6

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   BIRC6 gRNA (BIRC6 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_003-sgBIRC6-5

   Plasmid

   #202439

   Purpose

   Expression of a guide RNA targeting BIRC6

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   BIRC6 gRNA (BIRC6 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Donor_YTHDF2_KO_Neo

   Plasmid

   #186672

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Neomycin (YTHDF2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Donor_ANKRD1_KO_Hygro

   Plasmid

   #186668

   Purpose

   Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO Hygromycin (ANKRD1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACE-Truncated-Kir3.2-StreptagII

   Plasmid

   #177263

   Purpose

   Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

   Insert

   G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
   

   Tags

   StreptagII

   Expression

   Insect

   Mutation

   Residues 49-378

   Promoter

   polyhedrin promoter

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVD1 tRNA-gRNA E4-En-1 (GB2243)

   Plasmid

   #160565

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tRNA-gRNA position E4-En-1 (Multiplexing Edit)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA targeting human SLC12A5 gene stop codon

   Plasmid

   #158577

   Purpose

   For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

   Depositor

   Rudolf Jaenisch

   Article

   Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

   Insert

   sgRNA targeting human SLC12A5 gene stop codon
   

   Use

   CRISPR

   Tags

   Cas9 and GFP

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3Basic-ME.2/ApoEpromoter

   Plasmid

   #51436

   Purpose

   This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

   Insert

   APOE apolipoprotein E (APOE, ME2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   The ME.2 enhancer is fused upstream of the ApoE g…

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFBOH-MHL.RING1B

    Plasmid

    #224712

    Purpose

    Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    RING1b (RNF2 Human)
    

    Tags

    6x histidine tag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid

    #111731

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q145 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

    Plasmid

    #214138

    Purpose

    Insect cell expression of human 20S CP alpha-subunits

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    PSMA5 (PSMA5 Human)
    PSMA6 (PSMA6 Human)
    PSMA7 (PSMA7 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109

    Plasmid

    #111730

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q109 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-RPS6KA5

    Plasmid

    #23758

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    RPS6KA5 (RPS6KA5 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCMVtrunc F-tractinN9-39-msfGFPΔN7

    Plasmid

    #231552

    Purpose

    Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    F-tractin (Itpka Rat)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMVtrunc msfGFPΔC9-LifeactΔN2

    Plasmid

    #231556

    Purpose

    Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Lifeact (ABP140 Budding Yeast)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30

    Plasmid

    #111744

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q30 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-Puro-PNPT1

    Plasmid

    #223315

    Purpose

    Lentiviral vector expressing human PNPT1

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid

    #58477

    Purpose

    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Arginine 62 to Aspartic Acid

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42

    Plasmid

    #111746

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q42 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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