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pBa. BDNF-Halo

Plasmid

#162718

Purpose

Mammalian expression of rat BDNF

Depositor

Marvin Bentley

Publication

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Brain derived neurotrophic factor (Bdnf Rat)

Tags

Halo

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta actin

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.JARID2

Plasmid

#125166

Purpose

Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Publication

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

JARID2 (JARID2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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JB84

Plasmid

#128908

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Publication

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab9AQL-BirA*-HA-MAO (RAB9A Human)

Expression

Mammalian

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMH220

Plasmid

#122836

Purpose

dCas9 with GreenGate B-C flanking sequences

Depositor

Markus Schmid

Publication

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Mutation

Contains D10A and H840A mutations

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P25

Plasmid

#120036

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-sfGFP

Plasmid

#103983

Purpose

Plasmid encoding sfGFP

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

sfGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1+_LN-HA-AGO2-WT

Plasmid

#92009

Purpose

Expresses Lambda-N-HA-AGO2 (WT)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

Lambda-N-HA

Expression

Mammalian

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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KG371-GFP-mios

Plasmid

#87052

Purpose

mammalian expression of mios

Depositor

David Sabatini

Publication

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

mios (MIOS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-ITFG2

Plasmid

#87049

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Publication

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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bio-CD4d3+4-TMEM24

Plasmid

#73092

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding TMEM24 with bio-rat CD4d3+4

Depositor

Gavin Wright

Publication

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TMEM24 (C2CD2L Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pETpRha-alpha-SkTm

Plasmid

#72929

Purpose

Allows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoter

Depositor

Dan Mulvihill

Publication

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha skeletal Tropomyosin

Expression

Bacterial

Promoter

rhaB

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSC-hTDG-PreE2

Plasmid

#52272

Purpose

Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

Ubc9-GST

Expression

Bacterial

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P1-4A

Plasmid

#51252

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848 mutated to Ala

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pEMS1540

Plasmid

#29262

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple69 (FEZF2 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available since

Nov. 16, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5 HA-Arp8

Plasmid

#19796

Depositor

Joan Conaway, Ronald Conaway

Publication

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Arp8 (ACTR8 Human)

Tags

2XHA

Expression

Mammalian

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pB1H2w2-12-Oct1

Plasmid

#18041

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

HD of Oct1

Expression

Bacterial

Mutation

the homeodomain of Oct1 is fused to fingers 1 and…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe human Bmf

Plasmid

#17240

Depositor

Joan Brugge

Publication

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (BMF Human)

Use

Retroviral

Expression

Mammalian

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-EAF pBacPAK8

Plasmid

#15323

Depositor

Joan Conaway, Ronald Conaway

Publication

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor (EAF1 Human)

Use

Baculovirus construction

Tags

6x His and FLAG

Promoter

polyhedrin

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-C/EBPbZIP (pGEX-bZIPa)

Plasmid

#12435

Depositor

Dong-Er Zhang

Publication

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

68 a.a. fragment of C/EBP alpha (CEBPA Human)

Tags

GST

Expression

Bacterial

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
814 pSG5L HA PTEN 1-336

Plasmid

#10742

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

Amino acids 1-336.

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only