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Showing: 881 - 900 of 13580 results

  5. pCRISPEY-GAL-Nat-ADE2

    Plasmid
    #232103
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232103-plasmid-map-sequence-id-461835

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  9. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid
    #128746
    Purpose
    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.
    Depositor
    David Bartel
    Article
    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do
    Insert
    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 point mutations disrupting the miR-7 site
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128746-plasmid-map-sequence-id-254125

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  10. TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)

    Plasmid
    #221997
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    DddA: S1330I, A1341V, N1342S, E1370K, T1380I
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221997-plasmid-map-sequence-id-438639

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  11. pcDNA3.1-mCherry-CyrCR1.miR16

    Plasmid
    #128747
    Purpose
    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.
    Depositor
    David Bartel
    Article
    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do
    Insert
    Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128747-plasmid-map-sequence-id-254135

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  12. pdRBFC-NS1-YN

    Plasmid
    #74723
    Purpose
    Express C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling
    Depositor
    Aiming Wang
    Article
    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    HA and YFP N-terminal half domain
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74723-plasmid-map-sequence-id-146367

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  13. pdRBFC-NS1-YC

    Plasmid
    #74724
    Purpose
    Express C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling
    Depositor
    Article
    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    FLAG and YFP C-terminal half domain
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74724-plasmid-map-sequence-id-367138

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  15. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  16. pdRBFC-B2-YN

    Plasmid
    #74721
    Purpose
    Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling
    Depositor
    Aiming Wang
    Article
    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    HA and YFP N-terminal half domain
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74721-plasmid-map-sequence-id-329048

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  18. pdRBFC-B2-YC

    Plasmid
    #74722
    Purpose
    Express C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling
    Depositor
    Aiming Wang
    Article
    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    FLAG and YFP C-terminal half domain
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74722-plasmid-map-sequence-id-329052

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  19. Fireworks RFP[PTC+] (AVA2626)

    Plasmid
    #85444
    Purpose
    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85444-plasmid-map-sequence-id-171527

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  20. Fireworks GFP[PTC-] (AVA2598)

    Plasmid
    #85445
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85445-plasmid-map-sequence-id-171491

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Showing: 881 - 900 of 13580 results