We narrowed to 2,318 results for: pET28

1. pET28a-His6-UROD-N367A

   Plasmid

   #236740

   Purpose

   Protein overexpression of human uroporphyrinogen decarboxylase N367A

   Depositor

   Christina Woo

   Article

   Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

   Insert

   Uroporphyrinogen decarboxylase human N367A (UROD Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   N367A

   Promoter

   Available since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-His6-GFP-UROD

   Plasmid

   #236741

   Purpose

   Protein overexpression of eGFP-human uroporphyrinogen decarboxylase

   Depositor

   Christina Woo

   Article

   Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

   Insert

   eGFP-Uroporphyrinogen decarboxylase human (UROD Human, Synthetic)
   

   Use

   Tags

   His6 and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-SUMO-FLAG-TeKaiC

   Plasmid

   #229222

   Purpose

   For expression of T. elongatus KaiC protein

   Depositor

   Andy LiWang

   Insert

   kaiC (kaiC T. elongatus)
   

   Use

   Tags

   SUMO-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b-SUMO-SeKaiB-FLAG

   Plasmid

   #229219

   Purpose

   For expression of S. elongatus KaiB protein

   Depositor

   Andy LiWang

   Insert

   kaiB (kaiB S. elongatus)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-hfYFP-TEV-Bcx

   Plasmid

   #191195

   Purpose

   Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Bcx fusion
   

   Use

   Tags

   6xHis and Bacillus circulans xylanase

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-hfYFP-TEV-mScarlet-i

   Plasmid

   #191196

   Purpose

   Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & mScarlet-I fusion
   

   Use

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-hfYFP-TEV-SAV

   Plasmid

   #191197

   Purpose

   Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-LSSmGFP-TEV-mScarlet-i

   Plasmid

   #191199

   Purpose

   Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & mScarlet-I fusion
   

   Use

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-LSSmGFP-TEV-SAV

   Plasmid

   #191200

   Purpose

   Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28.His.3C.86b.8D2Q.Tau2N4R

    Plasmid

    #226400

    Purpose

    Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 8D2Q mutant 2N4R tau for recombinant protein production in bacteria

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau (MAPT synthetic, Human)
    

    Use

    Tags

    His.3C.86b

    Expression

    Bacterial

    Mutation

    8D2Q.Tau2N4R

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28.His.3C.86b.20D3Q.Tau2N4R

    Plasmid

    #226401

    Purpose

    Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteria

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT synthetic, Human)
    

    Use

    Tags

    His.3C.86b

    Expression

    Bacterial

    Mutation

    20D3Q, Tau2N4R

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-PKP3(305-797)

    Plasmid

    #202599

    Purpose

    Expresses ARM domain of plakophilin3

    Depositor

    Tina Izard

    Article

    Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

    Insert

    plakophilin3 (PKP3 Human)
    

    Use

    Tags

    His8 tag with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1 to 304

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

    Plasmid

    #187708

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-380

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a(+)-NEDD4L_W3-W4-HECT (492)

    Plasmid

    #187709

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-a(+)-6xHis-miRFP(-5)-HO1

    Plasmid

    #219424

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Inserts

    miRFP682
    HO1
    

    Use

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    Contains Ribosomal Binding Site for expression of…

    Promoter

    T7

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-FKBP-IRSp53(pLxIS)

    Plasmid

    #221336

    Purpose

    Overexpression of IRSp53 pLxIS domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    IRSp53 pLxIS domain (Baiap2 Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-FKBP-MAVS(pLxIS)

    Plasmid

    #221335

    Purpose

    Overexpression of MAVS pLxIS domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    MAVS pLxIS domain (Mavs Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-FKBP-TRIF(Nterm)

    Plasmid

    #221334

    Purpose

    Overexpression of TRIF Nterm domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    TRIF Nterm domain (Ticam1 Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-FKBP-GMIP(pLxIS)

    Plasmid

    #221337

    Purpose

    Overexpression of GMIP pLxIS domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    GMIP pLxIS domain (Gmip Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-SnoopTag-SpyTag-(AffiHER2)x3

    Plasmid

    #216281

    Purpose

    Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-SpyTag-(AffiHER2)x3
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge