Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

2,172 results

Showing: 1321 - 1350 of 2172 results

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pENTR-D-Topo-kcnk12

Plasmid

#208995

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk12 (kcnk12 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk13a

Plasmid

#208996

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk13a (kcnk13a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T1_gRNA

Plasmid

#199387

Purpose

pDEL111; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type I sgRNA

Use

Tol2 destination vector

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T2_gRNA

Plasmid

#199388

Purpose

pDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type II sgRNA

Use

Tol2 destination vector

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Zebrafish IntS6

Plasmid

#198410

Purpose

Expresses FLAG-tagged zebrafish IntS6

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

ints6 (ints6 Zebrafish)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Zebrafish IntS6L

Plasmid

#198411

Purpose

Expresses FLAG-tagged zebrafish IntS6L

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

ints6l (ints6l Zebrafish)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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mhc2dab:GFP-LT

Plasmid

#68996

Purpose

for making transgenic fish

Depositor

David Traver

Article

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

GFP-LT

Promoter

mhc2dab

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]

Plasmid

#198381

Purpose

Expression of SYPB::CRY2olig(535) in neurons of Danio rerio

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

UAS:sypb-egfp-cry2

Use

Tol2

Tags

EGFP

Mutation

D387A

Promoter

UAS

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T3_gRNA

Plasmid

#199389

Purpose

pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_erbb2_gRNA171

Plasmid

#199385

Purpose

pDEL133; Gateway Destination vector for cell-type specific CRISPR encoding erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

erbb2 sgRNA 171

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-Alk3

Plasmid

#207614

Purpose

Zebrafish Alk3 BMP receptor kinase domain fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

Alk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Vaucheria frigida, Zebrafish)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-BMPR2a

Plasmid

#207616

Purpose

Zebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

BMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Vaucheria frigida, Zebrafish)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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mhc2dab:mCherry

Plasmid

#68997

Purpose

for making transgenic fish

Depositor

David Traver

Article

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

mcherry

Promoter

mhc2dab

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb wnt3 promoter-Fzd1mApple

Plasmid

#203795

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

Insert

Fzd1 (fzd1 Zebrafish)

Use

Zebrafish expression

Tags

mApple

Promoter

4kb Wnt3 promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb wnt3 promoter-PMTmApple

Plasmid

#203794

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

Insert

PMT

Use

Zebrafish expression

Tags

mApple

Promoter

4kb Wnt3 promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Bmp7

Plasmid

#201909

Purpose

in vitro transcription of RNA encoding FLAG-tagged Bmp7a

Depositor

Mary Mullins

Article

Little et al Nat Cell Biol. 2009 May;11(5):637-43. do

Insert

bmp7 (bmp7a Zebrafish)

Tags

FLAG tag

Promoter

SP6

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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acvr1l-VAAA-HA

Plasmid

#201905

Purpose

in vitro transcription of RNA encoding HA-tagged Acvr1l that contains 4 amino acid substitutions: T186V, S187A, S189A, S191A

Depositor

Mary Mullins

Article

Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

acvr1l (acvr1l Zebrafish)

Tags

HA

Mutation

T186V, S187A, S189A, S191A

Promoter

SP6

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_typeEGF_gRNA

Plasmid

#199386

Purpose

pDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 EGF sgRNA

Use

Tol2 destination vector

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T2_gRNA

Plasmid

#197647

Purpose

pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type II sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
zCREST3:Cas9green; T1_gRNA

Plasmid

#199332

Purpose

pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type I sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-TOPO-DrSmg1_F

Plasmid

#146277

Purpose

Bacterial Expression of DrSmg1

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrSmg1 (smg1 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrSmg5_F

Plasmid

#146278

Purpose

Bacterial Expression of DrSmg5

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrSmg5 (smg5 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-TOPO-DrSmg6_F

Plasmid

#146279

Purpose

Bacterial Expression of DrSmg6

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrSmg6 (LOC100334020 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrSmg7_F

Plasmid

#146280

Purpose

Bacterial Expression of DrSmg7

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrSmg7 (smg7 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-TOPO-DrUPF1_F

Plasmid

#146281

Purpose

Bacterial Expression of DrUPF1

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF1 (upf1 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-TOPO-DrUPF2_F

Plasmid

#146282

Purpose

Bacterial Expression of DrUPF2

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF2 (upf2 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-TOPO-DrUPF3a_F

Plasmid

#146283

Purpose

Bacterial Expression of DrUPF3a

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF3a (upf3a Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-TOPO-DrUPF3b_F

Plasmid

#146284

Purpose

Bacterial Expression of DrUPF3b

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF3b (upf3b Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-tubcDNA_D

Plasmid

#146140

Purpose

Mammalian Expression of tubcDNA

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-tubcDNA-PTC33_D

Plasmid

#146141

Purpose

Mammalian Expression of tubcDNA-PTC33

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC33

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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