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pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_CBF-GLuc_L3-L2
Plasmid#113868PurposeSingle pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.DepositorInsertsCBF (notch dependent promoter)
GLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-N2-PKCgamma
Plasmid#21204DepositorInsertPKC gamma (Prkcg Rat)
UseTagsGFPExpressionMammalianMutationPKC gamma (rat). (Lab plasmid WC 0013)PromoterAvailable sinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-NtC60αESNtLC60β
Plasmid#160878PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli.DepositorInsertrbcL, cpn60α, cpn60β, groES
UseTagsExpressionBacterialMutationPromoterrbcL: T7, chaperones: T7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
Plasmid#113864PurposeSingle pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.DepositorInsertsPTCH1 (hedgehog dependent promoter)
mTurquoise2
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
UseTags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)
Plasmid#213034Purposefusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogeneticsDepositorInsertmNeonGreen, ISN-IE, ChRmine
UseAAVTagsGaussia luciferase signal sequenceExpressionMutationPromoterhSynAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6011nes
Plasmid#108652PurposeAn ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-ERK-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBB75-ncRNA
Plasmid#194087PurposeExpresses Ec86 ncRNA in Escherichia coliDepositorInsertEc86 ncRNA
UseTagsExpressionMutationPromoterT7Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6013x
Plasmid#108654PurposeAn HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertRaichu-EV
UseLentiviralTagsXFPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_TOP-NLuc1.1_L5-L4
Plasmid#113867PurposeSingle pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.DepositorInsertsTOP (wnt dependent promoter)
NLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Brachyury-eGFP
Plasmid#21063DepositorInsertBrachyury promoter
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'-t7- YFP
Plasmid#186564PurposepMB1' ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
anxA9-GFP
Plasmid#107198PurposeExpresses human annexin A9-GFP fusion protein for live cell imagingDepositorInsertannexin A9 (ANXA9 Human)
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6020nes
Plasmid#108656PurposeA JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-JNK-EV
UseTagsXFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ensconsin-18-mEos2
Plasmid#99230PurposeMammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.DepositorInsertmEos2
UseTagsEnsconsin (MAP7)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdUA-lacIN
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…TagsExpressionBacterialMutationPromoterEscherichia coli lacIN promoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272
Plasmid#22732DepositorInsertLox71-MCS-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE containi…PromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only