We narrowed to 31,881 results for: Eng

1. pCHC042 (∆phaCAB)

   Plasmid

   #226208

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for phaCAB
   

   Use

   Synthetic Biology

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

   Plasmid

   #73404

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   At4CL
   PhCHS
   CmCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Keasling-2337

   Plasmid

   #100168

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   lacIq-Ptrc-ADS
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZ_P-GAP-eGFP

   Plasmid

   #126717

   Purpose

   yeast plasmid overexpressing eGFP

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   egfp
   

   Expression

   Yeast

   Promoter

   GAP

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFNC-3

   Plasmid

   #233296

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Inserts

   BxbI phage integrase
   sacB
   

   Expression

   Bacterial

   Promoter

   PEM7 and unknown

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZ_P-AOX1-eGFP

   Plasmid

   #126718

   Purpose

   yeast plasmid overexpressing eGFP

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   egfp
   

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2340_Tier3(SB)-iRFP_Puro

   Plasmid

   #169638

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven iRFP670 and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS2362_Tier3(SB)-rtTA-PuroR

   Plasmid

   #169658

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHC005 (∆RE)

   Plasmid

   #226199

   Purpose

   Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for type I restriction-modification system restriction subunit H16_A0006
   

   Use

   Synthetic Biology

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCHC039 (∆phcA)

    Plasmid

    #226207

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for PhcA
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCA24N-ligase-cTF

    Plasmid

    #87743

    Purpose

    IPTG-inducible expression of ligase-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and cTF (chimeric transcription factor base…

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid

    #169548

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbE1k-3

    Plasmid

    #71722

    Purpose

    tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase (QDPR Human)
    pterin-4a-carbinolamine dehydratase (PCBD1 Human)
    

    Promoter

    pTrc

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB3K3 containing mRFP1

    Plasmid

    #118083

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, mRFP1
    

    Use

    Escherichia coli

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB1K3 containing mRFP1

    Plasmid

    #118082

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, mRFP1
    

    Use

    Escherichia coli

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pK_P-GAP-ACC1*

    Plasmid

    #126740

    Purpose

    yeast plasmid overexpressing ACC1 from Komagataella phaffii

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    acc1*
    

    Expression

    Yeast

    Mutation

    T3452G

    Promoter

    GAP

    Available Since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. asPink chromoprotein promoter-less

    Plasmid

    #118005

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, asPink
    

    Use

    Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9-beta-mmr

    Plasmid

    #169241

    Purpose

    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    mutL-E36K
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed glutamic acid 36 to lysine

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p168

    Plasmid

    #62618

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZ_lacO-cP-AOX1-NpgA

    Plasmid

    #126731

    Purpose

    yeast plasmid overexpressing NpgA

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    npgA
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    G951A,C957A

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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