We narrowed to 1,848 results for: Ipp

1. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

   Plasmid

   #71237

   Purpose

   Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A GFP
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX333

   Plasmid

   #64073

   Purpose

   Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   3XFLAG-Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CBh; U6

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124428

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Adeno EA

   Plasmid

   #64071

   Purpose

   Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Insert

   U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
   

   Use

   Adenoviral

   Tags

   FLAG-Cas9

   Expression

   Mutation

   Promoter

   U6 and CBh

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCCL/IFNB1-d2eGFP-3’UTR

   Plasmid

   #180232

   Purpose

   Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

   Insert

   d2eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   1 kb upstream of IFNB1 CDS

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmax pona 12xTRICK 24xMS2SL

   Plasmid

   #64542

   Purpose

   TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   12x PP7 stem-loops
   

   Use

   Tags

   24xMS2 stem loops, HA, NLS, and dNP799

   Expression

   Mammalian

   Mutation

   Promoter

   ponA

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1a-mMLH1dn

   Plasmid

   #174825

   Purpose

   Mammalian expression of mouse MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   mouse MLH1dn (codon optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 758-760

   Promoter

   EF1a

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-synP-FLEX-splitTVA-EGFP-B19G

   Plasmid

   #52473

   Purpose

   Expresses splitTVA-EGFP-B19G

   Depositor

   Ian Wickersham

   Article

   Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

   Has Service

   AAV1

   Insert

   TVA-P2A-EGFP-P2A-B19G
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin-1

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7-Elongin C-pcDNA3

    Plasmid

    #19998

    Depositor

    William Kaelin

    Article

    Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

    Insert

    Elongin C (ELOC Human)
    

    Use

    Tags

    T7-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV7.1_3xFLAG-TAOK2

    Plasmid

    #172992

    Purpose

    constitutive expression of FLAG-tagged TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK2 (TAOK2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmScarlet-HSP70

    Plasmid

    #163790

    Purpose

    Expresses mScarlet-Hsp70 in mammalian cells

    Depositor

    Vincent Timmerman

    Article

    Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

    Insert

    HSP70 (HSPA1A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET-pr-Kaede (Kaede-A69T)

    Plasmid

    #99228

    Purpose

    Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-Kaede
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV7.1_3xFLAG-TAOK1

    Plasmid

    #172991

    Purpose

    constitutive expression of FLAG-tagged TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK1 (TAOK1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti CAG-FLAG-dCas9-VPR

    Plasmid

    #114197

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPV-EF1a-mRxxFP1(AfeI)-iP-A

    Plasmid

    #204625

    Purpose

    Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-HA-MST2

    Plasmid

    #33098

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    MST2 (STK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200402

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid

    #200410

    Purpose

    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor detecting iRFP720
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV2-SynTetOff-GFP

    Plasmid

    #190231

    Purpose

    AAV vector, high-level expression of EGFP in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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