Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,485 results for: U6 promoter

Clear keywords
Showing: 881 - 900 of 1485 results

  2. pSc1-DD

    Plasmid
    #80439
    Purpose
    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    E. coli dihydrofolate reductase
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80439-plasmid-map-sequence-id-151455

    Enlarge

  8. pSc1-puro

    Plasmid
    #80438
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV, U6, and hPGK
    Available Since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80438-plasmid-map-sequence-id-151453

    Enlarge

  9. pVD1 tRNA-gRNA position E3 (GB2240)

    Plasmid
    #160562
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tRNA-gRNA position E3 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160562-plasmid-map-sequence-id-319301

    Enlarge

  11. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

    Plasmid
    #240865
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240865-plasmid-map-sequence-id-482051

    Enlarge

  12. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

    Plasmid
    #240094
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240094-plasmid-map-sequence-id-482052

    Enlarge

  13. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid
    #201677
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette
    Depositor
    Timo Otonkoski
    Article
    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-
    Insert
    MIR302-3g-EEA-2g-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201677-plasmid-map-sequence-id-395330

    Enlarge

  14. pVD1 tRNA-gRNA E4-En-1 (GB2243)

    Plasmid
    #160565
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tRNA-gRNA position E4-En-1 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160565-plasmid-map-sequence-id-319679

    Enlarge

  15. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

    Enlarge

  19. CBSH3- 3m shRNA3

    Plasmid
    #160213
    Purpose
    Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Mutation
    TGTATGACCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available Since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    160213-plasmid-map-sequence-id-314924

    Enlarge

  20. CBSH3- 3m shRNA2

    Plasmid
    #160211
    Purpose
    Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Mutation
    TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available Since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160211-plasmid-map-sequence-id-313962

    Enlarge

Showing: 881 - 900 of 1485 results