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Plasmid#204311PurposeFor generating targeting inversions. N-terminal split GFP, expressed in eyes.DepositorInsert3XP3-GFPn
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDHA
Plasmid#232987PurposeGalactose iduced expression of Gcn4 DBDAHA in yeastDepositorInsertDBD
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 L123A
Plasmid#232969PurposeGalactose iduced expression of Gcn4 L123A in yeastDepositorInsertGcn4 L123A
UseTagsTEV cleavage siteExpressionYeastMutationL123APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap2
Plasmid#232965PurposeGalactose iduced expression of Gcn4 k0.19 in yeastDepositorInsertGcn4 k0.19
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3
Plasmid#232960PurposeGalactose iduced expression of Gcn4 k0.47 in yeastDepositorInsertGcn4 k0.47
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120A
Plasmid#232962PurposeGalactose iduced expression of Gcn4 W120A in yeastDepositorInsertGcn4 W120A
UseTagsTEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBD
Plasmid#232985PurposeGalactose iduced expression of Gcn4 DBD in yeastDepositorInsertGcn4 DBD
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124I
Plasmid#232964PurposeGalactose iduced expression of Gcn4 F124I in yeastDepositorInsertGcn4 F124I
UseTagsTEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3HA
Plasmid#232998PurposeGalactose iduced expression of Gcn4 k0.47HA in yeastDepositorInsertGcn4 k0.47
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDGFP
Plasmid#232999PurposeGalactose iduced expression of Gcn4 DBDGFP in yeastDepositorInsertDBD
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS1
Plasmid#231615PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS1 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS9
Plasmid#231623PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS8
Plasmid#231622PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS6
Plasmid#231620PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS5
Plasmid#231619PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS4
Plasmid#231618PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS1 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS3
Plasmid#231617PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS2
Plasmid#231616PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 WT 30mer
Plasmid#231888PurposeBacterial expression of N-terminally 6His tagged Gcn4 WT 30merDepositorInsertGcn4 30mer
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_DBD
Plasmid#231886PurposeBacterial expression of N-terminally 6His tagged Gcn4 DBDDepositorInsertGcn4 DBD
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only