We narrowed to 1,028 results for: golden gate assembly

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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p1300-dCas9-HV5

Plasmid

#239479

Purpose

An intermediate vector is used to assemble three gRNAs via Golden Gate cloning.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

UBQ-dCAS9

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKG1334_pHarbor-PiggyBac_backbone

Plasmid

#239706

Purpose

PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated int

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK151

Plasmid

#183127

Purpose

BTK Type 4 reverse promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse CP25 promoter and forward rpoC terminator for dsRNA expression

Use

Synthetic Biology

Available Since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK150

Plasmid

#183126

Purpose

BTK Type 2 promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression

Use

Synthetic Biology

Available Since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Article

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Expression

Bacterial and Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAcCAST-sgRNA_entry (CJT83)

Plasmid

#181785

Purpose

Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pShoCAST-sgRNA_entry (BO1)

Plasmid

#181786

Purpose

Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pNM3746

Plasmid

#154109

Purpose

loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCALNL-BsaI-EGFP

Plasmid

#123136

Purpose

Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

EGFP, mRFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

cBA

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFUS_A8

Plasmid

#99882

Purpose

Recipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

TALEN

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pKG0893_pHarbor-LentiX1_backbone

Plasmid

#239704

Purpose

PaqCI Golden Gate assembly plasmid encoding the LentiX1 backbone

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pKG1179_pPV1-EFS

Plasmid

#239690

Purpose

BsaI Golden Gate assembly plasmid encoding the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EFS promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKG0586_pPV2-mRuby2

Plasmid

#239695

Purpose

BsaI Golden Gate assembly plasmid encoding the mRuby2 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2039_pPV1-CAG

Plasmid

#239686

Purpose

BsaI Golden Gate assembly plasmid encoding the CAG promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

CAG promoter

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHelper_ShCAST_sgRNA

Plasmid

#127921

Purpose

Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMC-Dest-BsaI

Plasmid

#200550

Purpose

Mini circle destination vector with BsaI sites for Golden Gate Assembly

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCEC-red

Plasmid

#196040

Purpose

Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

Depositor

Paola Branduardi

Article

Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Grna cloning

Expression

Yeast

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pShHELIX_sgRNA_entry (CJT32)

Plasmid

#181782

Purpose

Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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