We narrowed to 1,405 results for: puc57

1. pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169667

   Purpose

   Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   vanillic acid-controlled SEAP and iRFP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   VanO2-PCMVmin

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-Cj-sgRNA

   Plasmid

   #89753

   Purpose

   U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cj-SgRNA
   

   Use

   Sgrna expression under u6 promoter

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRExT2A-NT-PmNG

   Plasmid

   #232200

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC-fFuCas9-HTBNLS-hph

   Plasmid

   #121092

   Purpose

   Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

   Depositor

   Xiao-Jun Ji

   Article

   Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

   Insert

   Cas9 and hygromycin resist gene
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   gpdA;trpC

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. iCAP-ROOT

   Plasmid

   #192262

   Purpose

   AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

   Depositor

   Li Ye

   Article

   Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

   Insert

   Synthetic construct for ROOT VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2340_Tier3(SB)-iRFP_Puro

   Plasmid

   #169638

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven iRFP670 and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas12j-8-crRNA

   Plasmid

   #194967

   Purpose

   The plasmid expresses Cas12j-8-crRNA expression elements.

   Depositor

   Yongming Wang

   Article

   Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRExT2A-CT-BmNG

   Plasmid

   #232207

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRExT2A-CT-PmNG

   Plasmid

   #232201

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid

    #169658

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRExT2A-CT-PmSc

    Plasmid

    #232549

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGE332

    Plasmid

    #153241

    Purpose

    M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDGE333

    Plasmid

    #153242

    Purpose

    M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE335

    Plasmid

    #153244

    Purpose

    M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGE334

    Plasmid

    #153243

    Purpose

    M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGE337

    Plasmid

    #153246

    Purpose

    M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TC1154

    Plasmid

    #164876

    Purpose

    Split GFP reporter plasmid expressing GFP (b11beta

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    Split GFP(beta11)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TC1541A

    Plasmid

    #164875

    Purpose

    Split GFP reporter expressing GFP(beta1-10) following C>U editing of the target site

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    Split GFP(beta1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

    Plasmid

    #205447

    Purpose

    human AAVS1-targeting plasmid for expression of mouse CR1

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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