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pEMS1111

Plasmid

#29040

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple49 (DBH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1532

Plasmid

#29259

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple61 (DRD1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Nov. 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1376

Plasmid

#29177

Purpose

Expression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple89 (GFAP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1526

Plasmid

#29256

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple55 (DCX Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Nov. 7, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1267

Plasmid

#29139

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple225 (TBR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pAG146 RGS7 3'UTR

Plasmid

#12004

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N11 3'UTR (RGS7 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG143 AK024929 3'UTR

Plasmid

#12001

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N8 3'UTR (LINC00341 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pKJ-1

Plasmid

#11333

Depositor

Michael McBurney

Article

Adra et al Gene. 1987 . 60(1):65-74.

Insert

pgk-1 5' and 3' regions (Pgk1 Mouse)

Expression

Bacterial

Available Since

March 10, 2006

Availability

Academic Institutions and Nonprofits only
pRSET OCaMP

Plasmid

#224932

Purpose

Expresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope)

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Tags

6xHis, RSET peptide, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSLQ3538 eforRed-targeting sgRNA

Plasmid

#239455

Purpose

guide for eforRed

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting eforRed

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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ptet-lux-Tn7T-Gm

Plasmid

#226434

Purpose

This vector features a tetracycline-inducible system to regulate luxABCDE reporter expression, optimized for efficiency in P. aeruginosa.

Depositor

Xue Liu

Article

Zhang et al Nat Commun. 2025 Jul 1;16(1):5870. doi:

Inserts

optimized TetR
luxABCDE

Expression

Bacterial

Promoter

Pcon and Ptet

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX2

Plasmid

#237637

Purpose

For overexpression of mEGFP-SS18-SSX2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX2 (SS18, SSX2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Vgf-T2A-mCherry-CW3SL

Plasmid

#223661

Purpose

Expresses VGF and mCherry in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

Vgf-T2A-mCherry

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DD-T6B-MUT-YFP

Plasmid

#235144

Purpose

Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused mutant T6B peptide

Use

AAV

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

hSyn

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-U6-sgGAA

Plasmid

#232724

Purpose

Cas9 guide RNA targeting GAA repeats for A-to-G base editing

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

SpCas9 gRNA targeting GAA repeats

Expression

Mammalian

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-U6-sgCTG

Plasmid

#232723

Purpose

SpCas9 guide RNA targeting CAG repeats for C-to-T base editing

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

SpCas9 gRNA targeting CAG repeats

Expression

Mammalian

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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NPFF2-DuET

Plasmid

#213352

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

NPFF2 (NPFFR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-Vinculin_TurboID_Venus

Plasmid

#200212

Purpose

Lentivirus vector expressing vinculin with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

Vinculin_TurboID_Venus

Use

Lentiviral

Promoter

T7

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAK-1

Plasmid

#210131

Purpose

knock out BAK in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Bcl-2 homologous antagonist/killer (BAK1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP

Plasmid

#199456

Purpose

Expression of stdPCP-PH in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdPCP-PH

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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