We narrowed to 15,736 results for: LEA

1. pKP1416

   Plasmid

   #167643

   Purpose

   Express FepA T17C . Please note that mutation numbers are not based on start codon of insert.

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   fepA T17C
   

   Expression

   Bacterial

   Mutation

   fepA with T17C mutation (Mutation is T39C based o…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKP1583

   Plasmid

   #167645

   Purpose

   Express FepA D34C . Please note that mutation numbers are not based on start codon of insert.

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   fepA D34C
   

   Expression

   Bacterial

   Mutation

   fepA with D34C mutation (Mutation is D56C based o…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKP1382

   Plasmid

   #167647

   Purpose

   Express FepA G54C . Please note that mutation numbers are not based on start codon of insert.

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   fepA G54C
   

   Expression

   Bacterial

   Mutation

   fepA with G54C mutation (Mutation is G76C based o…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP718

   Plasmid

   #167636

   Purpose

   Express FepA T13C . Please note that mutation numbers are not based on start codon of insert.

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   fepA T13C
   

   Expression

   Bacterial

   Mutation

   fepA with T13C mutation (Mutation is T35C based o…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKP1410

   Plasmid

   #167634

   Purpose

   Express FepA D12C . Please note that mutation numbers are not based on start codon of insert.

   Depositor

   Kathleen Postle

   Article

   Postle et al bioRxiv 2021.12.08.471779

   Insert

   fepA D12C
   

   Expression

   Bacterial

   Mutation

   FepA with D12C mutation. (Numbering based on star…

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 24 pCOLI_A_N_STREP_C_FKBP

   Plasmid

   #160260

   Purpose

   N-term 2xStrepII 3C cleavable, C-term FKBP Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   2xStrepII and FKBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP89b CvTA

   Plasmid

   #141204

   Purpose

   Chromobacterium violaceum transaminase with TEV cleaving site after the N-terminal Histag.

   Depositor

   Francesca Paradisi

   Article

   Cerioli et al J Mol Cat B: Enz 2015 v120 (141-150)

   Insert

   CvTA
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

   Plasmid

   #119229

   Purpose

   Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.

   Depositor

   Kari Alitalo

   Insert

   IL3SP-Vegfd-dNdC (Vegfd Mouse)
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTAT6

   Plasmid

   #112594

   Purpose

   Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-thioredoxin fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6xHis-thioredoxin

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTAT5

    Plasmid

    #112593

    Purpose

    Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-thioredoxin fusions

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6xHis-thioredoxin

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. STK10 (STK10A-c043)

    Plasmid

    #110255

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    STK10 (STK10 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVP16-tev-PRT1-C195A

    Plasmid

    #83408

    Purpose

    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    C195A substitution

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVP16-tev-PRT1-C29A

    Plasmid

    #83407

    Purpose

    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    C29A substitution

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVP16-tev-PRT1-V28A

    Plasmid

    #83406

    Purpose

    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    V28A substitution

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. mKOet-LaminB1-10

    Plasmid

    #57928

    Purpose

    Localization: Nuclear Envelope, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mKOet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

16. PP-MAPK2 (3RP9)

    Plasmid

    #32862

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MAPK2
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    amino acids 123-548 only, P421L

    Available Since

    Jan. 10, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pBSG01

    Plasmid

    #37558

    Depositor

    Wei-Lih Lee

    Article

    Tang et al J Cell Biol. 2012 Mar 19;196(6):743-56.

    Insert

    PreScission protease cleavage site-S tag-TEV site-Z
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. TDRD7 (3RCO)

    Plasmid

    #32051

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TDRD7 (TDRD7 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    March 21, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pLIC.B3.CT0478

    Plasmid

    #11526

    Depositor

    Sung-Hou Kim

    Article

    Kim et al Proteins. 2006 Feb 1. 62(2):322-8.

    Insert

    ScpB
    

    Tags

    HisTag, TEV cleavable

    Expression

    Bacterial

    Available Since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pSAB35

    Plasmid

    #175787

    Purpose

    Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

    Depositor

    John Rubinstein

    Article

    Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

    Insert

    SidK (1-278)
    

    Tags

    3xFLAG and 6xHis TEV

    Expression

    Bacterial

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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