Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 53,418 results for: plasmid

Showing: 8981 - 9000 of 53418 results

  1. pT7-NSP3SA11-F2A-EGFP

    Plasmid
    #220808
    Purpose
    Express separately the Rotavirus SA11 NSP3 protein and the original enhanced GFP (Yang et al., 1996)
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 NSP3-F2A-EGFP
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220808-plasmid-map-sequence-id-443927

    Enlarge

  2. pFastBac HTB His-TEV-rDPP9(S729A)

    Plasmid
    #217327
    Purpose
    Purification of catalytically-dead rat DPP9 from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Use
    Tags
    His-TEV
    Expression
    Insect
    Mutation
    S729A
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217327-plasmid-map-sequence-id-433438

    Enlarge

  3. pET41-FeoB-C675S

    Plasmid
    #216756
    Purpose
    Investigation of the potential interaction of the FeoB Cys675 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C675 is substituted with S.
    Promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216756-plasmid-map-sequence-id-434497

    Enlarge

  4. pET41-FeoB-C429S

    Plasmid
    #216755
    Purpose
    Investigation of the potential interaction of the FeoB Cys429 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C429 is substituted with S.
    Promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216755-plasmid-map-sequence-id-434494

    Enlarge

  5. pET41-FeoB-D123N

    Plasmid
    #216754
    Purpose
    Production of a FeoB that is unable to bind GTP for a negative control in GTPases assays
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid D123 is substituted with N.
    Promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216754-plasmid-map-sequence-id-434490

    Enlarge

  6. L0-T12 GG2-ER-tagBFP-tUBQ10-GG3

    Plasmid
    #219695
    Purpose
    Level 0 to construct history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    GG2-ER-tagBFP-tUBQ10-GG3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219695-plasmid-map-sequence-id-435727

    Enlarge

  7. L0I75_tUBQ10_GG5-GG6

    Plasmid
    #219714
    Purpose
    Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG5-tUBQ10-GG6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT terminator with added golden gate linkers
    Promoter
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219714-plasmid-map-sequence-id-439236

    Enlarge

  8. pOCC102-MBP-eIF4G-SkA

    Plasmid
    #219977
    Purpose
    eIF4G-SkA protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-SkA (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    eIF4A binding domain of S. kudriavzevii
    Promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219977-plasmid-map-sequence-id-436800

    Enlarge

  9. pOCC102-MBP-eIF4G-RAR

    Plasmid
    #219973
    Purpose
    eIF4G-RAR protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-RAR (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Truncation: amino acids 490 to 942
    Promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219973-plasmid-map-sequence-id-436750

    Enlarge

  10. L0-T13 GG3-tRBCS-mCherry-NLS-GG4

    Plasmid
    #219696
    Purpose
    Level 0 to construct history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    GG3-tRBCS-mCherry-NLS-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219696-plasmid-map-sequence-id-435728

    Enlarge

  11. L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1

    Plasmid
    #219697
    Purpose
    Level 0 to construct history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-tUBQ1-NLS-NeonGreen-GG1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219697-plasmid-map-sequence-id-435859

    Enlarge

  13. L0I80_Bxb1mut(noBsaI)_GG1-GG4

    Plasmid
    #219715
    Purpose
    Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG1-Bxb1(noBsaI0)-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219715-plasmid-map-sequence-id-435740

    Enlarge

  14. hPLD3-sgRNA-Cas9_mcherry

    Plasmid
    #199342
    Purpose
    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199342-plasmid-map-sequence-id-390619

    Enlarge

  15. hPLD4-sgRNA-Cas9_mcherry

    Plasmid
    #199343
    Purpose
    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    sgRNA: accagtagtatgaagccacg
    Promoter
    human U6
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199343-plasmid-map-sequence-id-390620

    Enlarge

  16. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

    Enlarge

  17. pINER3R

    Plasmid
    #185883
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available Since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    185883-plasmid-map-sequence-id-430023

    Enlarge

  18. pLSU1/t35s[TMV]:mCherry:NosT

    Plasmid
    #212178
    Purpose
    This binary vector expresses mCherry with the truncated 35sCAMV promoter and Tomato Mosaic Virus UTR
    Depositor
    Wayne Curtis
    Insert
    mCherry
    Use
    Tags
    Expression
    Plant
    Mutation
    Truncated mCherry, missing the first 9 amino acid…
    Promoter
    truncated 35sCaMV
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212178-plasmid-map-sequence-id-422856

    Enlarge

  19. pLSU1/t35s[TMV]:MCS+:NosT

    Plasmid
    #212160
    Purpose
    This binary vector has an empty backbone with extended multiple cloning site (MSC) where you can inserta a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)
    Depositor
    Wayne Curtis
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212160-plasmid-map-sequence-id-422551

    Enlarge

  20. pLSU1/t35S[TMV]:mNeonGreen:NosT

    Plasmid
    #212162
    Purpose
    This binary vector expresses mNeonGreen with a truncated 35sCAMV promoter with strong UTR (Tomato Mosaic Virus)
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen
    Use
    Tags
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato
    Promoter
    truncated 35sCaMV
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212162-plasmid-map-sequence-id-422667

    Enlarge

Showing: 8981 - 9000 of 53418 results