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pGEX-NHERF-1-PDZ-1

Plasmid

#250116

Purpose

Bacterial expression of NHERF-1 PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

NHERF-1 (1/2) (Nherf1 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pYJ35-EZ-Tet-pLKO-shNKX3.1-Puro

Plasmid

#131078

Purpose

inducible NKX3-1 knock down

Depositor

Xiaojun Lian

Insert

NKX3.1 shRNA (NKX3-1 Human)

Use

RNAi

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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sgMARK2-3-1

Plasmid

#229440

Purpose

knockout of MARK2 and MARK3

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMARK2-3-1 (MARK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem S131C-wt)

Plasmid

#224777

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S131C

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem S131C-S131C)

Plasmid

#224779

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 S131C-S131C) (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S131C / S562C

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C)

Plasmid

#224778

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 wt-S131C) (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S562C

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem wt-wt)

Plasmid

#224776

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem S131C-S131C)

Plasmid

#224760

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / S562C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem wt-S131C)

Plasmid

#224759

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (wt-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S562C

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem S131C-wt)

Plasmid

#224758

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRevA-TEV-His12

Plasmid

#137034

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAF07416.1 (revA Borrelia burgdorferi B31)

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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MPS-1

Plasmid

#113057

Purpose

Expresses worm MPS-1 in mammalian cells

Depositor

Federico Sesti

Article

Bianchi et al J Biol Chem. 2003 Apr 4;278(14):12415-24

Insert

MiRP K+ channel accessory Subunit 1 1 (mps-1 Nematode)

Expression

Mammalian

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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FLT1 gRNA (BRDN0001146782)

Plasmid

#75558

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT1 (FLT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GAL FLAG103QDProGFP p306

Plasmid

#15834

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pET-3c-rhVTN-NC

Plasmid

#30226

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with N and C terminus cleavages (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 399-478, 20-62

Available Since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pRRL CAGpNFlag BAF155 ires GFP

Plasmid

#24561

Depositor

Jerry Crabtree

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

Insert

BAF155 (Smarcc1 Mouse)

Use

Lentiviral

Tags

Flag

Available Since

July 16, 2010

Availability

Academic Institutions and Nonprofits only
CAV1-K*R-HA

Plasmid

#27765

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

HA

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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Anti-PSD-95 [K28/43R]

Plasmid

#128618

Purpose

Mammalian Expression Plasmid of anti-PSD-95 (Human). Derived from hybridoma K28/43.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

Anti-PSD-95 (Human) recombinant mouse monoclonal antibody (DLG4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-P4Mx1

Plasmid

#108143

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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