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SF3B3-1

Plasmid

#156004

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SF3B3 (SF3B3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DDX11

Plasmid

#155502

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX11 (DDX11 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PPAPDC2

Plasmid

#155836

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPAPDC2 (PLPP6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero9

Plasmid

#187927

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero9

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero9

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-dddAI-FLAG

Plasmid

#186560

Purpose

Broad host range regulated expression of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Tags

FLAG

Expression

Bacterial

Promoter

tac

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pT7C-HSPA1L

Plasmid

#177660

Purpose

Recombinant protein synthesis of human HSPA1L

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSPA1L (HSPA1L Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9676-ABE

Plasmid

#162997

Purpose

plasmid expressing ABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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HSP70-pp7i-mCh-UPG

Plasmid

#161011

Purpose

C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_AAVS1_sgRNA_2

Plasmid

#155085

Purpose

lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab32a

Plasmid

#109621

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab32a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab32a (rab32a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EEA1

Plasmid

#109508

Purpose

Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EEA1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Gal4-entry

Plasmid

#128014

Purpose

Vector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

Gal4-DBD-3xFlag

Use

Gal4 entry vector

Tags

Gal4-DBD-3xFlag

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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bTub-GFP.766A

Plasmid

#112695

Purpose

expressed GFP in testes, under bTub promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

bTub-GFP-SV40

Expression

Insect

Promoter

bTub

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRC-1/1-IS1/2

Plasmid

#91349

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS1/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRC-1/1-IS2/2

Plasmid

#91251

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS2/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;lmo2P1Egfp

Plasmid

#90162

Purpose

contains lmo2 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2P1Egfp (lmo2 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.R132L

Plasmid

#81679

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway Cloning

Mutation

R132L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_p.R258H

Plasmid

#81581

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway Cloning

Mutation

R258H; p.588_589delLA-insRL

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SAMHD1_p.I515T

Plasmid

#81506

Purpose

Gateway Donor vector containing SAMHD1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SAMHD1 (SAMHD1 Human)

Use

Gateway Cloning

Mutation

G404E; I515T; A546V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.Q79K

Plasmid

#81479

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway Cloning

Mutation

Q79K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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