We narrowed to 9,648 results for: Ada;

1. pC0096 RESCUE truncation (Cas13 delta 892-1095)

   Plasmid

   #130663

   Purpose

   CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b(delta 892-1095)-ADAR2dd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-BICDN-CIBN

   Plasmid

   #102254

   Purpose

   Expresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   GFP-BICDN-CIB1 (CIB1 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. SaKKH-ABE8e

   Plasmid

   #138502

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSaCas9 (KKH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4-STRAD alpha

   Plasmid

   #14889

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   STRAD alpha (STRADA Human)
   

   Use

   Tags

   6xHis/Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-U6-sasgRNACMV- mCherry-WPREpA

   Plasmid

   #217015

   Purpose

   S. aureus sgRNAs backbone along with mCherry expression

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

   Plasmid

   #203229

   Purpose

   Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Park et al bioRxiv. 2023 Jun 2:2023.06.02.543490. d

   Insert

   Cre-dependent, membrane-localized Optopatch
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-SpdCas9-VP64

   Plasmid

   #115794

   Purpose

   Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   VP64
   

   Use

   AAV

   Tags

   3XFLAG and dCas9-VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. σ3-mCherry

   Plasmid

   #186580

   Purpose

   Expression of adaptor protein 3 (AP3) sigma subunit AP3S1 tagged with mCherry

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   AP3S1 (AP3S1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAS9id

   Plasmid

   #141251

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMK074: CMV QuasAr2-TS-GCaMP6f

    Plasmid

    #72303

    Purpose

    Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

    Depositor

    Adam Cohen

    Article

    Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

    Insert

    CaViar
    

    Use

    Lentiviral

    Tags

    TS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MT363

    Plasmid

    #65858

    Purpose

    The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

    Depositor

    Detlef Weigel

    Article

    Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

    Insert

    nahG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPEPY-PF6-lacI

    Plasmid

    #85589

    Purpose

    Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PF6

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAS9cd

    Plasmid

    #141252

    Purpose

    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. iSpyMac

    Plasmid

    #155014

    Purpose

    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

    Insert

    iSpyMac
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PID grafted onto SpyCas9 N-terminal domain, R221K…

    Promoter

    EF1ɑ

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2/CMV-Luc/PGK-SB100X

    Plasmid

    #192869

    Purpose

    Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

    Depositor

    David Largaespada, Maria Castro

    Article

    Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

    Inserts

    luc
    SB100X
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. hGATA-1 WT

    Plasmid

    #118352

    Purpose

    This plasmid expresses human GATA-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Plasmid

    #163076

    Purpose

    Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-AcGFP-progerin

    Plasmid

    #86947

    Purpose

    Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.

    Depositor

    Alex Hewitt

    Insert

    AcGFP-progerin (LMNA Human)
    

    Use

    AAV

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    progerin sequence is LMNA gene with nucleotide C1…

    Promoter

    CMV and T7

    Available since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9c

    Plasmid

    #141250

    Purpose

    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTJV1Sc-rpoB

    Plasmid

    #166978

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    Ec86 Retron (ECD_00831 )
    lambda bet
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101 and VanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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