We narrowed to 26,138 results for: Vit

1. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126758

   Purpose

   Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126759

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126753

   Purpose

   Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126754

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126756

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAWp63-clone3-12 (OptiHF-SpCas9)

   Plasmid

   #131737

   Purpose

   Expresses OptiHF-SpCas9 in mammalian cells

   Depositor

   Alan Siu Lun Wong

   Article

   Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

   Insert

   OptiHF-SpCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Q695A+K848A+E923M+T924V+Q926A

   Promoter

   EFS

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Met133I, M1381I, M186I ELL2

   Plasmid

   #127263

   Purpose

   For in vitro translation of human ELL2 with Met133I, M1381I, M186I

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Three Mets (133, 138, 186) to Ileu

   Promoter

   T7

   Available since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONRG_P4-P1R:TBA2p

   Plasmid

   #128429

   Purpose

   Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.

   Depositor

   George Haughn

   Article

   McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

   Insert

   TESTA ABUNDANT 2 (AT1G62060 Mustard Weed)
   

   Use

   Gateway promoter entry clone

   Tags

   Expression

   Mutation

   Promoter

   TESTA ABUNDANT 2

   Available since

   Oct. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. Designed HEL-binding scFv D44.1des

   Plasmid

   #111719

   Purpose

   Yeast Surface Display of D44.1des

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   D44.1 des
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…

   Promoter

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA tagged Sph ELL2

    Plasmid

    #127270

    Purpose

    For in vitro translation of human WT ELL2 with internal HA tag

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    WT Mets and HA tag at SphI site (8th Met)

    Promoter

    T7

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Met138ILeu ELL2

    Plasmid

    #127260

    Purpose

    For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met138 to Ileu Quikchange

    Promoter

    T7

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Met186ILeu ELL2

    Plasmid

    #127261

    Purpose

    For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met186 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met186 to Ileu Quikchange

    Promoter

    T7

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Met138ILeu, Met186ILeu ELL2

    Plasmid

    #127265

    Purpose

    For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Two Mets (138,186) to ILeu Quikchange

    Promoter

    T7

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Met133ILeu ELL2

    Plasmid

    #127259

    Purpose

    For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met133 to Ileu Quikchange

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Met133ILeu, Met186ILeu ELL2

    Plasmid

    #127264

    Purpose

    For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Two Mets (133,186 )to ILeu Quikchange

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Met331Leu ELL2

    Plasmid

    #127262

    Purpose

    For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met331 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met331 to ILeu Quikchange

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Met1ILeu ELL2

    Plasmid

    #127258

    Purpose

    For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met1 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met1 to Ileu by Quikchange

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ELL2mRNA+polyA

    Plasmid

    #127256

    Purpose

    For in vitro translation of human ELL2 with 30 nt pA tail

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    shortens 3'UTR to 40 bp adds A30 tail

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA tagged Xba ELL2

    Plasmid

    #127268

    Purpose

    For in vitro translation of WT ELL2 with HA tag inserted near N-term

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

    Promoter

    T7

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

20. pMCh1809_pBS_cdc42E6-boxB

    Plasmid

    #118609

    Purpose

    cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E6 3'UTR (Cdc42 Mouse)
    

    Use

    In vitro transcription

    Tags

    boxB

    Expression

    Mutation

    Promoter

    T3

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge