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Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector

Plasmid

#114429

Purpose

Target a Cre-dependent GFP cassette and Dre-dependent tTA2 cassette to the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-g0s2

Plasmid

#179294

Purpose

E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site

Depositor

Debra Hansen

Article

Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do

Insert

G0S2 (G0S2 Human)

Tags

mature maltose binding protein + enterokinase cle…

Expression

Bacterial

Mutation

full-length G0S2, residues 1-103 from accession n…

Promoter

Ptac

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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R929-X81-566

Plasmid

#246651

Purpose

Bacterial protein expression of His6-MBP-tev-Hs.HRAS(1-169) G13D

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Hs.HRAS(1-169) G13D (KRAS Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

G13D

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGMC00027

Plasmid

#199279

Purpose

SpCas9 construct to knockout murine Cd274

Depositor

Raj Chari

Article

Ruf et al Cell. 2023 Aug 17;186(17):3686-3705.e32.

Insert

Cd274 guide RNA (Cd274 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)

Plasmid

#190755

Purpose

Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.

Depositor

Dave Piston

Article

Foust et al Biophys J. 2025 Oct 21;124(20):3354-3365

Inserts

GNB1 (GNB1 Human)
GNG2 (GNG2 Human)
GNAI1 (GNAI1 Human)

Tags

mCherry2 and mEYFP(Q69K)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Ai139 (TIT2L-GFP-ICL-TPT) targeting vector

Plasmid

#114426

Purpose

Target a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP, tdTomato, tTA2,

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai170 (TIT2L-ASAP2s-Kv-ICL-tTA2) targeting vector

Plasmid

#114435

Purpose

Target a Cre-dependent voltage sensor ASAP2s cassette into the mouse TIGRE locus

Depositor

Michael Lin, Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ASAP2s-Kv, tTA2

Use

Cre/Lox and Mouse Targeting

Tags

Kv2.1 tag

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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R701-X52-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

Plasmid

#159560

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

Plasmid

#159539

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Ai167 (TIT2L-ChrimsonR-tdT-ICL-tTA2) targeting vector

Plasmid

#114431

Purpose

Target a Cre-dependent ChrimsonR-tdTomato cassette and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ChrimsonR-tdTomato, tTA2

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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