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pD649-HAsp-SEMA3G-COMP5AP-AviTag-9xHis

Plasmid

#157539

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3G (SEMA3G Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-SNAP-CD4-bla

Plasmid

#179447

Purpose

Donor vector to knock in SNAPtag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a del.SHD

Plasmid

#40036

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a del.SHD (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted SHD domain (1-143)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
EFNB2-tdTomato

Plasmid

#139967

Purpose

Donor vector to knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

Human EFNB2 knockin homology arms (EFNB2 Human)

Expression

Mammalian

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

Plasmid

#176838

Purpose

To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

sgPDX1

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp13 deltaN1-EGFP

Plasmid

#165127

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp13 deltaN2-EGFP

Plasmid

#165128

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-tet-puro-sh-ex16

Plasmid

#35167

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pSBbi-Pur-cpVenus[E172]

Plasmid

#125202

Purpose

SB-transposon plasmid for stable expression of cpVenus[E172] cDNA as a FRET single color control

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

cpVenus[E172]

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (T2 truncation)

Plasmid

#49211

Purpose

expresses Flag tagged mouse Tim3 (T2 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T2 truncation (Havcr2 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T2 truncation ( lacks all but one tyrosine, which…

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3 CCR5 HHE Short

Plasmid

#137697

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHE Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2AB

Plasmid

#40037

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2AB (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2A and C2B domains (353-671)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2A

Plasmid

#40038

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2A (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2A domain (353-487)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-mCerulean3-spacer-cpVenus[E172]

Plasmid

#125204

Purpose

SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET low control

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

mCerulean3-spacer-cpVenus[E172]

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-TPD54(L53P,L67P)

Plasmid

#172458

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutant

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

changed Leucines 53 and 67 to proline (breaks coi…

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (T1 truncation)

Plasmid

#49210

Purpose

expresses Flag tagged mouse Tim3 (T1 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T1 truncation (Havcr2 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T1 truncation ( contains all but the three most C…

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pEN_TT MEK5-DD-mRFP1

Plasmid

#47547

Purpose

Gateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MEK5-DD (MAP2K5 Human)

Use

Entry vector

Tags

mRFP

Mutation

S311D, T315D

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a C2B

Plasmid

#40039

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2B (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2B domain (488-671)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 hSlp2-a del.C2AB

Plasmid

#40059

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a del.C2AB (SYTL2 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion of C2A and C2B domains (601-910)

Promoter

Tac

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pGL3 CCR5 HHG Short

Plasmid

#137699

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHG Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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