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We narrowed to 15,990 results for: nes

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Showing: 9081 - 9100 of 15990 results

  2. CD8a-cNLS-EGFP

    Plasmid
    #86052
    Purpose
    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane along with two SV4…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86052-plasmid-map-sequence-id-177916

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  4. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

    Plasmid
    #50888
    Purpose
    Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50888-plasmid-map-sequence-id-76361

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  13. pTBL3440 MTK_PBdest lacO-hU6-BtoApegRNA-LacI-mCherry

    Plasmid
    #254862
    Purpose
    peCHYRON destination vector expressing IPTG-inducible B-to-A pegRNA, LacI, and mCherry. Can be integrated into the genome of mammalian cells with PiggyBac transposase.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d
    Inserts
    lacO_hU6_promoter-peCHYRON_BtoA_pegRNA
    pCAG-LacI-rb_glob_polyA
    pEF1alpha-mCherry-BGH_polyA
    Use
    Piggybac integration
    Expression
    Mammalian
    Available Since
    June 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254862-plasmid-map-sequence-id-512990

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  14. pTBL3439 MTK_PBdest lacO-hU6-AtoBpegRNA-LacI-mCherry

    Plasmid
    #254861
    Purpose
    peCHYRON destination vector expressing IPTG-inducible A-to-B pegRNA, LacI, and mCherry. Can be integrated into the genome of mammalian cells with PiggyBac transposase.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d
    Inserts
    lacO_hU6_promoter-peCHYRON_AtoB_pegRNA
    pCAG-LacI-rb_glob_polyA
    pEF1alpha-mCherry-BGH_polyA
    Use
    Piggybac integration
    Expression
    Mammalian
    Available Since
    June 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254861-plasmid-map-sequence-id-512989

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  15. CBP core-dCas9-CBP core

    Plasmid
    #179560
    Purpose
    encodes S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with both n-terminal and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179560-plasmid-map-sequence-id-365385

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  16. GCN5 FL-dCas9-CBP core

    Plasmid
    #179556
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179556-plasmid-map-sequence-id-354745

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  17. pLY152

    Plasmid
    #130950
    Purpose
    dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    PpspA-2G6 and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130950-plasmid-map-sequence-id-257362

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  18. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

    Plasmid
    #122955
    Purpose
    Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression
    Depositor
    Pierre Vanderhaeghen
    Article
    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.
    Insert
    NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
    Use
    Lentiviral
    Tags
    6xhis tag
    Mutation
    deletion of EGF repeats from full length NOTCH2NLB
    Promoter
    CAG promoter
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122955-plasmid-map-sequence-id-244415

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  19. pKG03572_pLentiX1-(TRE-mRuby2-bGH)-cHS4.core-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #252557
    Purpose
    Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 core insulator, lentiviral vector
    Depositor
    Kate Galloway
    Article
    Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.
    Insert
    rtTA-P2A-mGreenLantern
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EFS (human elongation factor 1-alpha - short/intr…
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    252557-plasmid-map-sequence-id-503968

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  20. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217780-plasmid-map-sequence-id-469426

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Showing: 9081 - 9100 of 15990 results