We narrowed to 31,954 results for: Eng

1. EGFP-PIK3R1

   Plasmid

   #174191

   Purpose

   Expresses PIK3R1 in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAJE-Ma pylRS nitroY/haloY-F5

   Plasmid

   #225684

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

   Depositor

   Ryan Mehl

   Inserts

   M. alvus PylRS 3-NY/HaloY F5
   M. alvus Pyl-tRNA(6)
   

   Use

   Methanomethylophilus alvus

   Mutation

   CTG125CTT, N166A, V168S, A223C, W239R

   Promoter

   GlnS and Lpp

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVC-Ds-MCS-Ds

   Plasmid

   #102416

   Purpose

   Empty vector with a multiple cloning site flanked by Ds integration elements

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET302-6His-dCas9-Halo

   Plasmid

   #72269

   Purpose

   Encodes a Halo-labeled fusion of nuclease deficient Cas9

   Depositor

   Timothée Lionnet

   Article

   Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

   Insert

   His6-dCas9-Halo
   

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHT-ads

   Plasmid

   #47382

   Purpose

   Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pgrac

   Available Since

   Oct. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK_Sox10_enh_84_Citrine

   Plasmid

   #130584

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   Sox10_enh_84
   

   Use

   Enhancer reporter

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2_8xCTS-ECFP

   Plasmid

   #200249

   Purpose

   Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   8xCTS-ECFP
   

   Use

   Zebrafish expression

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT09

   Plasmid

   #223381

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. gRNA_GFP-T1

   Plasmid

   #41819

   Purpose

   Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_GFP-T1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. p177bp-LacOx162-Hyg

    Plasmid

    #210215

    Purpose

    Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    162xLacO repeats derived from: PMID: 12864855.
    

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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