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pMRXIP-GFP-TRPML1(PHD)

Plasmid

#221752

Purpose

Stable expression of GFP-TRPML1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

TRPML1 (MCOLN1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem TRPML1(PHD) (amino acids 1–69)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ALDOA_sgRNA1

Plasmid

#201590

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

ALDOA (ALDOA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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p-Tet-O-Sox5-Puro

Plasmid

#221667

Purpose

Expresses Sox5 in mammalian cells

Depositor

Vanessa Hall

Article

Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

Insert

Sox5 (Sox5 Mouse)

Use

Lentiviral

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1b-3X FLAG

Plasmid

#218536

Purpose

human FLVCR1b with C-terminal 3X_FLAG cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

FLAG (3X)

Expression

Mammalian

Mutation

Alternative isoform

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG

Plasmid

#218534

Purpose

human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

FLAG (3X)

Expression

Mammalian

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_TMEM104

Plasmid

#132262

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

TMEM104 (TMEM104 Human)

Expression

Mammalian

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC11A2

Plasmid

#131884

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC11A2 (SLC11A2 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_UCP2

Plasmid

#132286

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UCP2 (UCP2 Human)

Expression

Mammalian

Available Since

Nov. 13, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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GFP(N-glyc)-Tac-TC

Plasmid

#162496

Purpose

Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

partial length Tac (TMD and cytosolic tail) (IL2RA Human)

Tags

GFP

Expression

Mammalian

Mutation

Tac is in partial length with its TMD and cysotol…

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A6

Plasmid

#132153

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A6 (SLC39A6 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC30A7

Plasmid

#132148

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A7 (SLC30A7 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SV2B

Plasmid

#132281

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SV2B (SV2B Human)

Expression

Mammalian

Available Since

May 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAcBac3-EcTrp-TGA-EGFP**

Plasmid

#228786

Purpose

EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTrpRS
EcWtR
EGFP**

Use

Baculoviral

Expression

Mammalian

Mutation

TGA suppressor

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A30_STOP

Plasmid

#161218

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A30 (SLC25A30 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLCO2A1

Plasmid

#131949

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO2A1 (SLCO2A1 Human)

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC25A24

Plasmid

#131980

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A24 (SLC25A24 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC46A2

Plasmid

#132203

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC46A2 (SLC46A2 Human)

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC9A6

Plasmid

#132175

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A6 (SLC9A6 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti TMEM30A

Plasmid

#171789

Purpose

transfer plasmid for lentiviral vector production expressing TMEM30A

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

TMEM30A (TMEM30A Human)

Use

Lentiviral

Tags

6X His + Flag

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC17A6

Plasmid

#131948

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A6 (SLC17A6 Human)

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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