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FHUG+W (B2)

Plasmid

#74011

Purpose

lentiviral expression of EGFP and shRNA (from H1 promoter, empty)

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7xTS-mPlum

Plasmid

#117392

Purpose

Tn7 tagging vector pTn7xTS with mPlum expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

mPlum

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pJY3919

Plasmid

#113332

Purpose

Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Expression

Yeast

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLN382 (FuGW-S(HIF1A)p-mKate2 )

Plasmid

#105180

Purpose

synthetic promoter HIF1A drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(HIF1A)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-EGFP-Bulged-miR-19

Plasmid

#91976

Purpose

EGFP reporter cell line with eight bulged miR-19 sites

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSBDEST.N

Plasmid

#79468

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Neomycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-IRES-EGFP-L2

Plasmid

#32591

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

IRES-EGFP

Use

Gateway Cloning

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pBbS6k-RFP

Plasmid

#35294

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbB6a-GFP

Plasmid

#35355

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbS1a-RFP

Plasmid

#35337

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Trc

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbA1c-RFP

Plasmid

#35335

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Trc

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbA6a-RFP

Plasmid

#35289

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mPax1 (CT#1039)

Plasmid

#13902

Depositor

Cliff Tabin

Article

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

Pax1 (Pax1 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pDONR223 SARS-CoV-2 NSP5 Cys146Ala_nostop

Plasmid

#154030

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5

Use

Gateway-compatible entry vector, with mutagenized…

Mutation

Many synonymous changes due to codon optimization

Available Since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV_53BP1_FLAG

Plasmid

#237345

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Insert

53BP1 (TP53BP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC18

Plasmid

#231666

Purpose

Expresses human ZDHHC18 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC18 (ZDHHC18 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC2

Plasmid

#231512

Purpose

Expresses human ZDHHC2 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC2 (ZDHHC2 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-HaloGFP-Na2.4

Plasmid

#228471

Purpose

Mammalian expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

Plasmid

#219826

Purpose

Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

Depositor

Philipp Maass

Type

Empty backbone

Expression

Mammalian

Promoter

EF1a

Available Since

July 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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KW78_HART_ODC1_PuroR

Plasmid

#207401

Purpose

Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR - PuroR

Depositor

Stephen Floor

Article

Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

mini CMV

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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