We narrowed to 3,182 results for: UBC

1. pAAVTREGCaMP6f

   Plasmid

   #97410

   Purpose

   Amplified expression of GCaMP6f in combination with TET driver

   Depositor

   Tetsuo Yamamori

   Article

   Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. HaloTag-3xrGBD

   Plasmid

   #176108

   Purpose

   Visualization of active Rho

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   rhotekin (Rtkn Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVdel

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROPseq-Guide-BSD

   Plasmid

   #216123

   Purpose

   Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3_V5-APEX2-HMOX1

   Plasmid

   #85824

   Purpose

   Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   V5-APEX2-HMOX1 (HMOX1 Synthetic, Human)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUB6-CDC34-HA (pRKB261)

   Plasmid

   #99145

   Purpose

   Mammalian expression construct to express HA-tagged CDC34

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   CDC34 (CDC34 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   pTight

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-1 3xFlag-CREB

   Plasmid

   #82721

   Purpose

   Expresses human CREB

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   CREB (CREB1 Human)
   

   Use

   Tags

   3XFLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

   Plasmid

   #182825

   Purpose

   Cre-dependent expression of H2B-APEX fusion

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   H2B.APEX-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lck-mTurquoise2-iLID

   Plasmid

   #176125

   Purpose

   Plasma membrane localized iLID

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   iLID
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304-CAR-V5

   Plasmid

   #82723

   Purpose

   Expresses V5 tagged coxsackievirus and adenovirus receptor (CAR).

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   coxsackievirus and adenovirus receptor (CXADR Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pCDFDuet-MKK6-EE

    Plasmid

    #82718

    Purpose

    Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

11. pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin

    Plasmid

    #134864

    Purpose

    Expresses EGFP-tagged nuclear envelope marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    emerin (EMD Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDUET-GST AO7REwt

    Plasmid

    #213935

    Purpose

    GST tagged AO7 encoding aa 126-258 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    AO7 cloned into the first multiple cloning site o…

    Promoter

    T7 promoter-1

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-4T-1 3xFlag-MAPKAPK2

    Plasmid

    #82720

    Purpose

    Expresses human MAPKAPK2

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MAPKAPK2 (MAPKAPK2 Human)
    

    Use

    Tags

    3xFLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pSG5-His-SUMO1

    Plasmid

    #17271

    Depositor

    Stephen Goff

    Article

    Yueh et al J Virol. 2006 Jan . 80(1):342-52.

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3_HMOX1-V5-APEX2

    Plasmid

    #85823

    Purpose

    Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    HMOX1-V5-APEX2 (HMOX1 Synthetic, Human)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)mGAT1-0-GFP

    Plasmid

    #41662

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal hydrophobic isoleucine residue added a…

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3_V5-APEX2-Sec61B

    Plasmid

    #83412

    Purpose

    Expressed N-term tagged APEX2 on Sec61B in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    V5-APEX2-Sec61B (SEC61B Synthetic, Human)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-EF1a-Neuromodulin-IRES-Puromycin

    Plasmid

    #134666

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Neuromodulin (GAP43 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T-1 3xFlag-STAT1

    Plasmid

    #82722

    Purpose

    Expresses human STAT1

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Tags

    3xFLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

20. pBT3024

    Plasmid

    #122542

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_mt::CemOrange2
    

    Use

    Tags

    MLSLRQSIRFFKPATRTLCSSRTLL

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLX302-MKP5-V5 puro

    Plasmid

    #87770

    Purpose

    Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV-Lck-FRB-mTurquoise2

    Plasmid

    #176130

    Purpose

    Plasma membrane localized FRB, rapamycin heterodimerization

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    FRB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MAC_C_EZRI

    Plasmid

    #111665

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    EZRI (EZR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLX302-JSP1-V5 puro

    Plasmid

    #87773

    Purpose

    Expresses V5 tagged human JSP1 (DUSP22)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    JSP1 (DUSP22 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

    Plasmid

    #182826

    Purpose

    Cre-dependent expression of membrane localized APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    LckAPEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MAC_C_LAMP1

    Plasmid

    #111669

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TIRAP-GFP

    Plasmid

    #52739

    Purpose

    Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

28. LightR-iCre-miRFP670

    Plasmid

    #162158

    Purpose

    Expresses LightR-iCre-miRFP670 in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-iCre-miRFP670
    

    Use

    Tags

    NLS and miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MAC_C_ARP2

    Plasmid

    #111658

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    ARP2 (ACTR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCX-SponGee-Kras

    Plasmid

    #134777

    Purpose

    mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

    Insert

    SponGee-Kras
    

    Use

    Tags

    FLAG and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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