We narrowed to 4,591 results for: ado

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EF1a_MEF2C_P2A_Hygro_Barcode

Plasmid

#120458

Purpose

Barcoded lentiviral vector to express MEF2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF13_P2A_Hygro_Barcode

Plasmid

#120499

Purpose

Barcoded lentiviral vector to express KLF13 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF13 (KLF13 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF3_P2A_Hygro_Barcode

Plasmid

#120490

Purpose

Barcoded lentiviral vector to express KLF3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF3 (KLF3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2

Plasmid

#196526

Purpose

Entry vector for Gateway with Eps8L2

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2-FL (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4RA-P2A-Puro

Plasmid

#112672

Purpose

Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4RA

Use

Lentiviral

Tags

FLAG

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf ctnna1L344P

Plasmid

#185407

Purpose

Zebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

ctnna1 (ctnna1 Zebrafish)

Use

In vitro synthesis of mrna

Expression

Mammalian

Mutation

changed Leucine 344 to Proline

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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LoxP2-Mb2Tomato-2A (SO240)

Plasmid

#99614

Purpose

To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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LoxP2-H2B-EGFP-V5-2A (SO107)

Plasmid

#99617

Purpose

To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3RA-P2A-GFP-PGK-Puro

Plasmid

#110868

Purpose

Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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LoxP1-Mb2YFP-2A (SO244)

Plasmid

#99613

Purpose

To clone gene of interest downstream of LoxP1-Mb2YFP-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2EYFP

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF10_P2A_Hygro_Barcode

Plasmid

#120496

Purpose

Barcoded lentiviral vector to express KLF10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF10 (KLF10 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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LoxP3-HA-H2B-Cerulean-2A (SO104)

Plasmid

#99618

Purpose

To clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA10_P2A_Hygro_Barcode

Plasmid

#120452

Purpose

Barcoded lentiviral vector to express HOXA10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA10 (HOXA10 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF9_P2A_Hygro_Barcode

Plasmid

#120495

Purpose

Barcoded lentiviral vector to express KLF9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF9 (KLF9 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-PGK-Puro

Plasmid

#110860

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_ASCL3_P2A_Hygro_Barcode

Plasmid

#120428

Purpose

Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL3 (ASCL3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA11_P2A_Hygro_Barcode

Plasmid

#120453

Purpose

Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA11 (HOXA11 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_KLF11_P2A_Hygro_Barcode

Plasmid

#120497

Purpose

Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF11 (KLF11 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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LoxP3-Mb2-HA-mTFP1-2A (SO157)

Plasmid

#99615

Purpose

To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-P2A-Puro

Plasmid

#110853

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ctdnep1_D67E-2xFlag

Plasmid

#196519

Purpose

Expression of Ctdnep1_D67E-2xFlag

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1_D67E-2xFlag (CTDNEP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

D67E mutation (phosphastase dead)

Promoter

CMV promoter; T7 promoter

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+CeGFP-Ctdnep1WTsiR

Plasmid

#196517

Purpose

Expression of Ctdnep1WT-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1WTsiR (CTDNEP1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

CMV promoter; T7 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXB6_P2A_Hygro_Barcode

Plasmid

#120454

Purpose

Barcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXB6 (HOXB6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 C1(259-443)

Plasmid

#89832

Purpose

Express GATA3 C1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

C1 (AA 259-443)

Promoter

CMV

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-P2A-Puro

Plasmid

#110851

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF1-MCS2-EF1-Puro-RGS7

Plasmid

#113728

Purpose

Express RGS7 in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP Kind2 S181E

Plasmid

#105312

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S181E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181A

Plasmid

#105307

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S181A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PA-mCit-NL

Plasmid

#113452

Purpose

Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine-NanoLuc

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

hUBbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

Plasmid

#184406

Purpose

yeast surface display of the SARS-CoV-2 Beta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

K417N+E484K+N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_HOXA1_P2A_Hygro_Barcode

Plasmid

#120451

Purpose

Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA1 (HOXA1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
His 3C Kinase dead MKK6 1-334

Plasmid

#87882

Purpose

E. coli expression of MKK6 kinase dead

Depositor

Peter Mace

Article

Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

MKK6 (MAP2K6 Human)

Tags

3C protease site and 6xHis

Expression

Bacterial

Mutation

K82A, kinase dead mutant

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-VQRRA-PGK-Puro

Plasmid

#110858

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Basic-Fascin-Promoter (-402-0)

Plasmid

#89823

Purpose

Promoter luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Promoter

SV40

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FRT2-H2B-EGFP-V5-2A (IG157)

Plasmid

#99623

Purpose

To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSP1-mp53AS-6xMUT

Plasmid

#20904

Depositor

Stefano Piccolo

Article

Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

Insert

p53AS (Trp53 Mouse)

Expression

Mammalian

Mutation

point mutation (Ser/Thr-Ala) of the 6 N-terminal …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
EF1a_KLF12_P2A_Hygro_Barcode

Plasmid

#120498

Purpose

Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF12 (KLF12 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-VRERRA-PGK-Puro

Plasmid

#110859

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1801 - 1840 of 4591 results

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We narrowed to 4,591 results for: ado