We narrowed to 5,032 results for: plasmid dna
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Plasmid#63754Purpose3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.DepositorInsertehxA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSc2
Plasmid#80437PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV-STAT31F
Plasmid#134725PurposeExpresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707DepositorUseTagsFLAG and human STAT3 aa 1-514 joined to human STA…ExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV-STAT13F
Plasmid#134723PurposeExpresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707DepositorUseTagsFLAG and human STAT1 aa1-508 joined to human STAT…ExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PrimeRoot.v2C-Cre
Plasmid#201739PurposeFor DNA insertion test in protoplastDepositorInsertePPE,Cre
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-trGluc
Plasmid#158959PurposeDNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).DepositorInserttrGluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-DNMT3a-DNMT3l
Plasmid#154140PurposeExpresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
UseTagsHA and sfGFPExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pASPIre3
Plasmid#154844PurposeDerivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this studyDepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-I-SceI
Plasmid#60960PurposePlasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesisDepositorInsertI-SceI restriction enzyme
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
phU6-gRNA
Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermouse U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman 7SKAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman H1Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
HP17_KO7
Plasmid#120346PurposeHelper plasmid for production of phagemid ssDNA, based on M13KO7DepositorInsertCoding part of the M13KO7 helper phage genome
UseTagsExpressionBacterialMutationPromoternative phage promotersAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
G421C_BattB(GA)_mCherry
Plasmid#200633PurposeStrong mCherry fluorescence recombination plasmid for Matreyek Bxb1(GA) landing padDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
HP4_M13
Plasmid#120340PurposeHelper plasmid for production of phagemid ssDNA, based on m13mp18DepositorInsertCoding part of the M13 phage genome
UseTagsExpressionBacterialMutationPromoternative phage promotersAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP
Plasmid#21373Purposeself-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMLRT
Plasmid#78234PurposeExpresses Murine Leukemia Reverse Transcriptase (MLRT)DepositorInsertMurine leukemia reverse transcriptase
UseTagsExpressionBacterialMutationPromoterJ23102Available sinceAug. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FDP-P10B2-A75-RZ-URA3
Plasmid#130874PurposeIntegration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tailDepositorInsertmKate
UseExpression of mkate, leu2(q180*), and ura3 on ort…TagsExpressionMutationPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AP1
Plasmid#179678PurposeAccessory plasmidsDepositorInsert[SD8] gIII
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pkk223_Null
Plasmid#213111Purposepkk223 backbone plasmidDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
G413A_BattB(GA)-EGFP
Plasmid#200632PurposeStrong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing padDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.26-SS-352
Plasmid#68791PurposeZF9 Op x6 -- GFP-IRES-NTRDepositorInsertsZF9 Op 6x
EGFP
Nitroreductase
UseTagsIRESExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
mitoZFD_ND1-Right-G1397-C
Plasmid#180769PurposeExpresses mitoZFD_ND1-Right-G1397-C(DddAtox half) in mammalian cellsDepositorInsertmitoZFD for ND1 mutation
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mitoZFD_ND1-Left-G1397-N
Plasmid#180768PurposeExpresses mitoZFD_ND1-Left-G1397-N(DddAtox half) in mammalian cellsDepositorInsertmitoZFD for ND1 mutation
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTGFP-T7-Hha10T
Plasmid#65427PurposeParent vector for constructing non-replicating lesion-bearing plasmidDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-delta8.2R
Plasmid#89706PurposeDonor plasmid for HIV-1 env- integration into human AAVS1 locusDepositorInsertAAVS1-delta8.2R
UseUnspecifiedTagsExpressionMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mitoZFD_ND2-Right-G1397-C
Plasmid#180771PurposeExpresses mitoZFD_ND2-Right-G1397-C(DddAtox half) in mammalian cellsDepositorInsertmitoZFD for ND2 mutation
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mitoZFD_ND2-Left-G1397-N
Plasmid#180770PurposeExpresses mitoZFD_ND2-Left-G1397-N(DddAtox half) in mammalian cellsDepositorInsertmitoZFD for ND2 mutation
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRTVIR
Plasmid#46365DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep
Plasmid#208644Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
UseCRISPR; Ssdna donnor preparationTagsTwin StrepExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
G1095C_BattB(GA)_jGCaMP7c_IRES_shBleR-P2A-HygroR
Plasmid#200638PurposejGCaMP7c recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cellsDepositorInsertjGCaMP7c
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSW1KN0022
Plasmid#197538PurposeMoClo Level 1 luciferase reporterDepositorInsertDREB26::LucN::FLAG::nosT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSW1KN0024
Plasmid#197539PurposeMoClo Level 1 luciferase reporterDepositorInsertNLP7::LucN::FLAG::nosT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSW1KN0025
Plasmid#197540PurposeMoClo Level 1 luciferase reporterDepositorInsertNIR1::LucN::FLAG::nosT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSW1KN0070
Plasmid#197536PurposeMoClo Level 1 luciferase reporterDepositorInsertCaMV35s::TMV::LucN::FLAG::35sT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSW1KN0016
Plasmid#197537PurposeMoClo Level 1 luciferase reporterDepositorInsertANR1::LucN::FLAG::nosT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBM10a
Plasmid#179688PurposeTarget plasmid for context profilingDepositorInsertACA target
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
CHD1 (CHD1A-c107)
Plasmid#110253PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCHD1 (CHD1 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7 - lacOAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only