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Showing: 2251 - 2300 of 18430 results

  2. pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2)

    Plasmid
    #231234
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    TAD deleted (Δ1-143), deletion of degron 1 & …
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231234-plasmid-map-sequence-id-459167

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  8. pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2

    Plasmid
    #169833
    Purpose
    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain
    Depositor
    Richard Possemato
    Article
    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422
    Insert
    CAD (CAD Human)
    Use
    Retroviral
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Deletions of amino acids R1398 - S1407 and F1366 …
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    169833-plasmid-map-sequence-id-339176

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  9. pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

    Plasmid
    #169835
    Purpose
    Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker
    Depositor
    Richard Possemato
    Article
    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422
    Insert
    CAD (CAD Human)
    Use
    Retroviral
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Substitution of amino acids F1308 - C1455 with a …
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    169835-plasmid-map-sequence-id-333093

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  10. pFSynW SYT9 D197, 199, 330, 332N IRES GFP

    Plasmid
    #195703
    Purpose
    Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter
    Depositor
    Edwin Chapman
    Article
    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.
    Insert
    Syt9 (Syt5 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    full-length mouse SYT9 with D197, 199, 330, 332N …
    Promoter
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195703-plasmid-map-sequence-id-383663

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  23. pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49227
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49227-plasmid-map-sequence-id-73643

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  30. pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

    Plasmid
    #141371
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Article
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    C145A, human codon optimized
    Promoter
    EF1alpha
    Available since
    April 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141371-plasmid-map-sequence-id-288079

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  39. RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

    Plasmid
    #139444
    Purpose
    Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    sequence codon-optimized
    Promoter
    SFFV
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139444-plasmid-map-sequence-id-308466

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  41. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

    Plasmid
    #122959
    Purpose
    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression
    Depositor
    Pierre Vanderhaeghen
    Article
    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.
    Insert
    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    Use
    Lentiviral
    Tags
    6xmyc tag
    Expression
    Mutation
    deletion of C terminus from full length NOTCH2NLB
    Promoter
    CAG promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122959-plasmid-map-sequence-id-244512

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  42. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

    Plasmid
    #122956
    Purpose
    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression
    Depositor
    Pierre Vanderhaeghen
    Article
    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.
    Insert
    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    Use
    Lentiviral
    Tags
    6xhis tag
    Expression
    Mutation
    deletion of C terminus from full length NOTCH2NLB
    Promoter
    CAG promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122956-plasmid-map-sequence-id-244423

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  49. pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

    Plasmid
    #231235
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    TAD deleted (delta1-143), DBD deleted (delta340-4…
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231235-plasmid-map-sequence-id-459171

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Showing: 2251 - 2300 of 18430 results