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pG1-NMGR526(d22-69)

Plasmid

#1125

Depositor

Susan Lindquist

Article

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

sup35 NM domain fused with GR (SUP35, Nr3c1 Rat, Budding Yeast)

Use

Tags

GR526

Expression

Yeast

Mutation

nucleotides encoding NM domain amino acids 22-69 …

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RNMT 1-120

Plasmid

#112706

Purpose

Expresses N-terminally FLAG-tagged human RNMT 1-120 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 121-476

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RNMT 121-476

Plasmid

#112707

Purpose

Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 2-120

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pJW1668

Plasmid

#112042

Purpose

CEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

FET3

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pJW1669

Plasmid

#112043

Purpose

CEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

NSG2

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pJW1672

Plasmid

#112046

Purpose

CEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

PST1

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pJW1674

Plasmid

#112048

Purpose

CEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SWP1

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-K1124EY1127EK1131E-dsRNAres_Y

Plasmid

#148077

Purpose

Insect Expression of DmPAN2-K1124EY1127EK1131E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-K1124EY1127EK1131E-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2-K1124EY1127EK1131E-dsRNAres_Y

Plasmid

#148046

Purpose

Bacterial Expression of DmPAN2-K1124EY1127EK1131E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-K1124EY1127EK1131E-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPKm-112

Plasmid

#90494

Purpose

pcDNA3 - pCMV - MTAD - PIF3, expresses Phytochrome interacting factor 3 (PIF3) and minimal transactivation domain (MTAD), under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

MTAD-PIF3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.112

Plasmid

#184981

Purpose

Express -Eco1 FAA1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12

Use

Tags

Expression

Yeast

Mutation

FAA1 donor P233stop

Promoter

Gal7

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRE112

Plasmid

#43828

Depositor

Dieter Schifferli

Article

Edwards et al Gene. 1998 Jan 30;207(2):149-57.

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb1

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pTP1122

Plasmid

#104159

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTP1112

Plasmid

#104158

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAF112

Plasmid

#135920

Purpose

All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Type

Empty backbone

Use

Luciferase; Transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRN1120

Plasmid

#101750

Purpose

Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

Depositor

Rene Verwaal

Article

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDD112

Plasmid

#119893

Purpose

pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pRDA_112

Plasmid

#136475

Purpose

Lentiviral expression of AsCas12a

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

AsCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pRN112

Plasmid

#84464

Purpose

pJB38-NWMN29-30+ SarA_P1-mAmetrine-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mAmetrine from pRN12

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB112

Plasmid

#68566

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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