We narrowed to 2,492 results for: ERK

1. pActin-miRFP670nano3

   Plasmid

   #184665

   Purpose

   Human beta-actin labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-human beta-actin
   

   Use

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. peCALWY-6

   Plasmid

   #22238

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY6 Zn(II) sensor (ATOX1 GFP's, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C416S mutation Identical to peCALWY-4, but linke…

   Promoter

   Available since

   Jan. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

3. anxA9-GFP

   Plasmid

   #107198

   Purpose

   Expresses human annexin A9-GFP fusion protein for live cell imaging

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Goebeler et al FEBS Lett. 2003 Jul 10;546(2-3):359-64.

   Insert

   annexin A9 (ANXA9 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. peCALWY-5

   Plasmid

   #22237

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY-5 Zn(II) sensor (ATOX1 GFP's, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C416S mutation identical to peCALWY-4, but linke…

   Promoter

   Available since

   Nov. 25, 2009

   Availability

   Academic Institutions and Nonprofits only

5. peCALWY-3

   Plasmid

   #22235

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY-3 Zn(II) sensor (ATOX1 GFP's, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Similar to eCALWY-1, but with a linkerlength of 3…

   Promoter

   Available since

   Nov. 20, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pKeratin-miRFP670nano

   Plasmid

   #127437

   Purpose

   Keratin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   Keratin-miRFP670nano
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Keratin V282L - please see depositor comments bel…

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. peCALWY-2

   Plasmid

   #22234

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY-2 Zn(II) sensor (ATOX1 GFP's, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence identical to eCALWY-1, but linkerlength …

   Promoter

   Available since

   Nov. 20, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pzyxin-emiRFP703

   Plasmid

   #136568

   Purpose

   Zyxin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   zyxin-emiRFP703
   

   Use

   Tags

   emiRFP703

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a Gx-NL

   Plasmid

   #169105

   Purpose

   Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGen2.1-redCALWY-4

    Plasmid

    #50738

    Purpose

    Expresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

    Insert

    gene for redCALWY-4 Zn2+ sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. piGECI-NES-N1

    Plasmid

    #160422

    Purpose

    Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGen2.1-redCALWY-1

    Plasmid

    #50739

    Purpose

    Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

    Insert

    gene for redCALWY-1 Zn2+ sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-G12-CheRiff-T2A-mCherry

    Plasmid

    #170275

    Purpose

    CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    CheRiff-T2A-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    11xUAS with TATA-box minimal promoter

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. YEpURA-CUP1-HBTubiquitin

    Plasmid

    #26900

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

    Insert

    HBT-tagged ubiquitin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. eDrTrkA

    Plasmid

    #183996

    Purpose

    Optically controlled eDrTrkA expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkA (NTRK1 Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a Gx-NL3

    Plasmid

    #169108

    Purpose

    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEB1deltaC-2xEGFP shRNA resistant

    Plasmid

    #37925

    Depositor

    Torsten Wittmann

    Article

    Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion after aa248, TTCCTGT=>TCCCGGT

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD-BpBBS

    Plasmid

    #199467

    Purpose

    Arabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.

    Depositor

    Vladislav Verkhusha

    Article

    Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

    Insert

    BpBBS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a Gx-NL2

    Plasmid

    #169107

    Purpose

    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLAMP1-miRFP718nano

    Plasmid

    #195749

    Purpose

    LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    LAMP1-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge