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pGC4785

Plasmid

#113409

Purpose

Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

Depositor

Guillaume Cambray, Adam Arkin

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Insert

359_31121113_2

Use

Synthetic Biology

Tags

fused to codon deptimized sfGFP with Flexible lin…

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-oChD-citrine

Plasmid

#50976

Purpose

AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

oChD-citrine

Use

AAV

Tags

citrine

Expression

Mutation

ChD gene is mammalian codon optimized

Promoter

human synapsin promoter

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pUB_SMFLuc0---MS2

Plasmid

#210568

Purpose

Expresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SMFLuc100---MS2

Plasmid

#210570

Purpose

Expresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFPO-His-G-Ca-FLITS

Plasmid

#191455

Purpose

Bacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

His

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2385-Tier1-PhCMV-fastFT

Plasmid

#169518

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven fluorescent timer, fast maturation time

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2384-Tier1-PhCMV-medFT

Plasmid

#169517

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT (PhCMV-medFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven fluorescent timer, medium maturation time

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

Plasmid

#173462

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)

Plasmid

#173460

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-mNeonGreen (pJJF003)

Plasmid

#173459

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

Plasmid

#173458

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 S727E

Plasmid

#12305

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Jun 26. ():.

Insert

STAT1 S727E (STAT1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S727E

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 S727A

Plasmid

#12304

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Jun 26. ():.

Insert

STAT1 S727A (STAT1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S727A

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pETM6-E12-vioABECD

Plasmid

#66537

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBHM2404

Plasmid

#173441

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-NAT1

Use

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBHM2406

Plasmid

#173442

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-HYGB

Use

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-mTAF6

Plasmid

#44337

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF6

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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IgK-IFN-luc

Plasmid

#14886

Purpose

Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

Depositor

David Baltimore

Article

Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

Insert

IgK-IFN (IFNB1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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pRSetB-KanR-LiLac

Plasmid

#184569

Purpose

Fluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expression

Depositor

Gary Yellen

Article

Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

Insert

LiLac fluorescent sensor of lactate

Use

Tags

His7

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSP2-96-merTetO-EFS-BLaR

Plasmid

#118713

Purpose

Contains an optimized 96-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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