We narrowed to 1,090 results for: cas12a

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pKT02_MSCV-hU6-BbsI-cPPT-EFS-Cre-mScarlet

Plasmid

#256628

Purpose

Retroviral vector for delivery of AsCas12a guides and expressing Cre and mScarlet using EFS promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

Retroviral

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pRK16b_MSCV-hU6P-LbDR-BbsI-cPPT-EFS-mScarlet

Plasmid

#256632

Purpose

Retroviral vector for delivery of LbCas12a guides and expressing mScarlet using EFS promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

Retroviral

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTF-ahpC-rfp

Plasmid

#153035

Purpose

For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

Depositor

Nigel Scrutton

Publication

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

CRISPR array

Expression

Bacterial

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pKT07_AAV-hU6-AsDR-crTSG1-EFS-Cre-T2A-hNGFR

Plasmid

#256630

Purpose

AAV vector for delivery of AsCas12a crTSG1 guide array and expressing Cre using EFS promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Insert

crTrp53, crPten, crApc, crRb1

Use

AAV

Promoter

humanU6, EFS-NS

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKT07_AAV-hU6-AsDR-EFS-Cre-T2A-hNGFR

Plasmid

#256629

Purpose

AAV vector for delivery of AsCas12a guides and expressing Cre and hNGFR using EFS promoter

Depositor

Sidi Chen

Publication

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

AAV

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pC1.530

Plasmid

#205441

Purpose

Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

Depositor

Alistair McCormick

Publication

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

RepB gRNA

Use

Synthetic Biology

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMV_AA089

Plasmid

#216105

Purpose

gRNA expression for Cas12a (guide only)

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gRNA expression

Use

CRISPR and Lentiviral; Assembled vector

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.391

Plasmid

#203948

Purpose

Level 0 part. Cas12a coding sequence from Francisella novicida in CDS1 position

Depositor

Alistair McCormick

Publication

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

FnCas12a

Use

Synthetic Biology

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_936

Plasmid

#216094

Purpose

Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD46, CD47, CD55, CD81 guides

Use

CRISPR and Lentiviral; Positive controls

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL089

Plasmid

#137897

Purpose

iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Publication

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL088

Plasmid

#137896

Purpose

iSTU-CRISPR/Cas12a DR-DR structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Publication

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER1-TF1-dsRED

Plasmid

#213044

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Publication

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

cBH

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER2-TF1-dsRED

Plasmid

#213045

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Publication

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

Plasmid

#202760

Purpose

Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

Depositor

Karl Wahlin

Publication

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

huAsCpf1

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL087

Plasmid

#137895

Purpose

iSTU-CRISPR/Cas12a guide RNA unit insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Publication

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pRDB_131

Plasmid

#216095

Purpose

Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD97, CD4, CD26, CD274 guides

Use

CRISPR and Lentiviral; Positive controls

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNHEJ-Cas9-recX

Plasmid

#158715

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

Depositor

Yi-Cheng Sun

Publication

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

cas9

Use

CRISPR

Expression

Bacterial

Promoter

TetO

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

Plasmid

#140203

Purpose

Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.

Depositor

Yit Heng Chooi

Publication

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

crRNA array elcA

Use

CRISPR and Synthetic Biology

Promoter

PgpdA

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RRV

Plasmid

#188543

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

Depositor

Yiping Qi

Publication

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RRV

Use

CRISPR; Gateway compatible lbcas12a-rrv entry clo…

Tags

NLS

Expression

Plant

Mutation

G146R, D156R, R182V

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YE1

Plasmid

#154145

Purpose

Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

Depositor

Jin-Soo Kim

Publication

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE1 (dCas12a-BE-YE1)

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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