We narrowed to 922 results for: cas12a

1. pY30

   Plasmid

   #84745

   Purpose

   Expresses huAsCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-LbCpf1-Blast

   Plasmid

   #84751

   Purpose

   Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   LbCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY095

   Plasmid

   #84744

   Purpose

   Expresses huLbCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   GFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAsCpf1(TATV)(BB) (pY221)

   Plasmid

   #89354

   Purpose

   Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1 RVR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K548V/N552R

   Promoter

   CBh

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3TS-zAsCpf1

   Plasmid

   #107185

   Purpose

   plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   AsCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mutation

   Promoter

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSP64T-hLbCpf1

   Plasmid

   #107187

   Purpose

   plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   hLbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mutation

   Promoter

   sp6

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_052_HygroR

   Plasmid

   #228937

   Purpose

   Plasmid for cloning of custom gRNA using EnAsCas12a

   Depositor

   Francisca Vazquez

   Article

   Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA048

   Plasmid

   #216016

   Purpose

   Fragmid fragment: (Cas protein) deactivated Cas

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   dCas12a (D908A)_v1.1 [EnAs]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY109 (lenti-LbCpf1)

    Plasmid

    #84740

    Purpose

    Lenti virus delivery of LbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY108 (lenti-AsCpf1)

    Plasmid

    #84739

    Purpose

    Lenti virus delivery of AsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. WN10150

    Plasmid

    #80443

    Purpose

    Expresses dead (908A) AsCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    nuclease dead (908A) AsCpf1
    

    Use

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    nuclease dead (D908A)

    Promoter

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid

    #104563

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1 (D832A)
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6His-MBP-TEV-huAsCpf1

    Plasmid

    #90095

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huAsCpf1
    

    Use

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6His-MBP-TEV-huLbCpf1

    Plasmid

    #90096

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huLbCpf1
    

    Use

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSN067

    Plasmid

    #101748

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDA052H_sgCh2

    Plasmid

    #229023

    Purpose

    Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA052H_sgFOCAD #2

    Plasmid

    #229024

    Purpose

    Expression of a EnAsCas12a guide targeting FOCAD

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    FOCAD gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid

    #104567

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    

    Use

    CRISPR

    Tags

    NLS-3xHA-VPR

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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