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Plasmid#127304PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/ACG-nLuc-3XFLAG
Plasmid#127303PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
miR-223-RFP pCDNA
Plasmid#97142Purposeexpression of miR-223 and TagRFPDepositorInsertmiR-223
UseTagsTagRFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS Exon Vector
Plasmid#69901PurposeExpression plasmid for expressing circular RNAs of a desired sequence in mammalian cellsDepositorInsertZKSCAN1 (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4
Plasmid#204692PurposeThis plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.DepositorInsertBRD4 (BRD4 Human)
UseTagsmNeonGreenExpressionMammalianMutationn/aPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi2-91-NLuc
Plasmid#207149PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI2 (GNAI2 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)QPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI3 (GNAI3 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt
Plasmid#157692Purposemammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-HCMVgO-sfGFP (TB40/E)
Plasmid#136451PurposeMammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFPDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsGly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-sfGFP (Merlin)
Plasmid#136452PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFPDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsGly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (TB40/E)
Plasmid#136445PurposeMammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-717). Codon-optimize…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (TB40/E)
Plasmid#136453PurposeMammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tagDepositorInsertenvelope glycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES EGFP
Plasmid#136620PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-G97-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-F98 IRES EGFP
Plasmid#136618PurposePlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-F98-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_SZ1-RGG-GFP-RGG
Plasmid#124932PurposeExpression of SZ1-RGG-GFP-RGGDepositorInsertSYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag
Plasmid#154970PurposeExpresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.DepositorInsertPfEBA175
UseTagsGFP and signal peptide (residues 1 to 32) from TG…ExpressionMammalianMutationinsert codes for EBA175 residues 1284 to 1462PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG
Plasmid#127322PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG
Plasmid#127323PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ER-EBFP2
Plasmid#231183PurposeExpresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cellsDepositorInsertER-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ER-mRFP
Plasmid#231176PurposeExpresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cellsDepositorInsertER-mRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only