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pLentiCRISPRpuro-sgSEC24C #1

Plasmid

#231563

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24C (#1) (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgSEC24C #1 (SEC24C Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF-1a

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-Necl5-ScNeo

Plasmid

#209901

Purpose

To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Necl5-ScNeo (Pvr Mouse)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbleo-TGFa-ScNeo

Plasmid

#209904

Purpose

To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-ScNeo (TGFA Human)

Use

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-TGFa-ScNeo

Plasmid

#209908

Purpose

To monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-ScNeo (TGFA Human)

Use

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-TGFa-ScNeo

Plasmid

#209895

Purpose

To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-ScNeo (TGFA Human)

Use

Lentiviral

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro XLone-CCND2

Plasmid

#179845

Purpose

donor plasmid for inducible expression of CCND2 in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA

Plasmid

#205442

Purpose

production of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminus

Depositor

Jeremy Kay

Article

Prigge et al Dev Cell. 2023 Oct 23;58(20):2080-2096.e

Insert

Unc5c (Unc5c Mouse)

Use

AAV

Tags

3xHA

Expression

Mutation

Promoter

mini-CMV (with MVM small intron)

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-dTLN1 intron

Plasmid

#176224

Purpose

A vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dTLN1 exon

Plasmid

#176033

Purpose

A vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluJo-Y 240-2996

Plasmid

#171131

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluSz 3501-4065

Plasmid

#171132

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluY WT 2598-2996

Plasmid

#171134

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluY-Sz WT 2598-4066

Plasmid

#171136

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF8_P2A_Hygro_Barcode

Plasmid

#120494

Purpose

Barcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF8 (KLF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hOKMS

Plasmid

#51543

Purpose

Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

Depositor

Tarjei Mikkelsen

Article

Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

Insert

hOKMS (MYC, KLF4, SOX2, POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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PB-TAC-ERP2-(N-HA)KRAS_G12D

Plasmid

#201254

Purpose

Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

Depositor

Alexander Kleger

Article

Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

Insert

KRAS (KRAS Human)

Use

Piggybac transposon

Tags

HA-Tag

Expression

Mammalian

Mutation

changed Gly (G) 12 to Asp (D)

Promoter

TRE

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-Hygro-2A-TMRPSS2

Plasmid

#170390

Purpose

Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

Depositor

Nir Hacohen

Article

Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

June 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pD649-HAsp-NEO1-COMP5AP-AviTag-9xHis

Plasmid

#157594

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NEO1 (NEO1 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1

Plasmid

#97043

Purpose

Expresses RUNX1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1 (RUNX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet on

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only