We narrowed to 4,969 results for: mcs

1. poRFP-oGFP

   Plasmid

   #154130

   Purpose

   Expresses orthogonal gfp and orthogonal rfp genes in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

   Inserts

   orthogonal gfp
   orthogonal rfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4_NFAT-RE-CMV-BC0547-luc2

   Plasmid

   #214665

   Purpose

   barcoded assay, Ca2+ sensor

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   6x clustered NFAT element with barcode 0547
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJBL7004

   Plasmid

   #136943

   Purpose

   Malachite green aptamer, T7 promoter

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

   Insert

   malachite green aptamer (MGA)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pT7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. poLuc1

   Plasmid

   #154128

   Purpose

   Expresses orthogonal firefly luciferase gene in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

   Insert

   orthogonal firefly luciferase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Salmonella MTAN

   Plasmid

   #64041

   Purpose

   Expression of S. enterica MTAN in E. coli

   Depositor

   Vern Schramm

   Article

   Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

   Insert

   Methylthioadenosine nucleosidase
   

   Tags

   Histidine tag

   Expression

   Bacterial

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCOLA-T7-WspR:D70E

   Plasmid

   #79164

   Purpose

   Expresses constitutively active WspR D70E under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   WspR (wspR P. areuginosa)
   

   Expression

   Bacterial

   Mutation

   D70E

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGH045_EGFP

   Plasmid

   #85411

   Purpose

   lentiviral expression vector for GFP

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJA327

   Plasmid

   #74486

   Purpose

   superfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)

   Depositor

   Andrew Fire

   Article

   Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

   Insert

   superfolder GFP
   

   Expression

   Worm

   Promoter

   myo-3

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETb-tVYN-TmΔ

   Plasmid

   #149694

   Purpose

   High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

   Depositor

   Ming Hammond

   Article

   Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

   Insert

   VenusΔC12-TmYcgRΔ-NLucΔ4
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4_NFAT-RE-CMV-BC0527-luc2

    Plasmid

    #214657

    Purpose

    barcoded assay, Ca2+ sensor

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    6x clustered NFAT element with barcode 0527
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCOLA-T7-WspR:G249A

    Plasmid

    #79163

    Purpose

    Expresses inactive WspR G249A under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    WspR (wspR P. areuginosa)
    

    Expression

    Bacterial

    Mutation

    G249A

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MBP-RGG-SmBit-RGG

    Plasmid

    #176644

    Purpose

    SmBit fused to LAF-1 RGG domain with N-terminal MBP tag

    Depositor

    Daniel Hammer

    Article

    Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

    Insert

    MBP-RGG-SmBit-RGG
    

    Expression

    Bacterial

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hy_pMT EGFP SV40 pA

    Plasmid

    #69911

    Purpose

    Expresses EGFP in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    EGFP
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJEC625

    Plasmid

    #174368

    Purpose

    Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4_CRE-CMV-BC0482-luc2

    Plasmid

    #214635

    Purpose

    barcoded assay, cAMP/Ca2+ sensor

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    6x clustered CRE element with barcode 0482
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJA317

    Plasmid

    #74488

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    d1ODC
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDUET-aaCPRct-A13AMO

    Plasmid

    #20116

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and Amorphadiene oxidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pJBL7032

    Plasmid

    #133873

    Purpose

    AtzR expression plasmid

    Depositor

    Julius Lucks

    Article

    Silverman et al ACS Synth Biol. 2020 Mar 20;9(3):671-677

    Insert

    AtzR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJA318

    Plasmid

    #74489

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::d4ODC::wPRE has d4ODC degron

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    d4ODC
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJBL7034

    Plasmid

    #133869

    Purpose

    AtzA expression plasmid

    Depositor

    Julius Lucks

    Article

    Silverman et al ACS Synth Biol. 2020 Mar 20;9(3):671-677

    Insert

    AtzA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge