We narrowed to 1,209 results for: proc.2

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Publication

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS

Plasmid

#248695

Purpose

Expression dead CRISPR/Cas9 with unnatural amino acid incorporated at K1151 residue

Depositor

Shuibing Chen

Publication

Zhu et al Proc Natl Acad Sci U S A. 2025 Oct 7;122

Insert

dead CRISPR/Cas9 K1151TAG, MbPylRS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-A*24:02

Plasmid

#235133

Purpose

E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*24:02 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*01:01

Plasmid

#235134

Purpose

E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*01:01 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDY279

Plasmid

#182959

Purpose

Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)

Depositor

Shelley Copley

Publication

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Insert

gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSN554

Plasmid

#177361

Purpose

Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

Depositor

Shinsuke Niwa

Publication

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Tags

Leucine zipper, StrepII tag, and mScarlet

Expression

Bacterial

Promoter

T7

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSN687

Plasmid

#177363

Purpose

Expresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tag

Depositor

Shinsuke Niwa

Publication

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Tags

His tag and Leucine zipper

Expression

Bacterial

Mutation

changed Proline 305 to Leucine

Promoter

T7

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N+D280N+D443N)

Plasmid

#134291

Purpose

Expresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteria

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acids 30, 280 and 443 to Asparag…

Promoter

T7

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v2

Plasmid

#134277

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 2)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

553 amino acids, isoform 2

Promoter

CMV

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D443N)

Plasmid

#134293

Purpose

Expresses GK5 (isoform 2)-D443N mutant in bacteria

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 443 to Asparagine

Promoter

T7

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N+D280N)

Plasmid

#134292

Purpose

Expresses GK5 (isoform 2)-D30N/D280N mutant in bacteria

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acids 30 and 280 to Asparagine

Promoter

T7

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D280N)

Plasmid

#134290

Purpose

Expresses GK5 (isoform 2)-D280N mutant in bacteria

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 280 to Asparagine

Promoter

T7

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N)

Plasmid

#134289

Purpose

Expresses GK5 (isoform 2)-D30N mutant in bacteria

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 30 to Asparagine

Promoter

T7

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-GK5_v2

Plasmid

#134280

Purpose

Expresses GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBOB-N-Flag-GK_v2

Plasmid

#134271

Purpose

Lentivector encoding Flag-tagged GK (isoform 2)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 2; 553 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-3HA-GK5_v2

Plasmid

#134269

Purpose

Lentivector encoding 3XHA-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v2

Plasmid

#134260

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES2-DsRed-MST-Kv2.1

Plasmid

#131707

Purpose

Expresses Kv2.1in mammalian cells

Depositor

Jennifer Kearney

Publication

Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

Insert

KCNB1 (KCNB1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJO428

Plasmid

#111141

Purpose

URT-type vector for Val-mIκBα expression in Mammalian cells

Depositor

Alexander Varshavsky

Publication

Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]

Plasmid

#66781

Purpose

Gly426 of miniSOG was mutated to 426Cys in tomm-20N::miniSOG

Depositor

Yishi Jin

Publication

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

tomm-20 (N terminal 55 aa)

Expression

Worm

Mutation

G426C

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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