Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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sgPlxnb2-1 in pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239588

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)

Use

Sleeping beauty (sb) transposon

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_ZIKV_NS5_MTase

Plasmid

#213529

Purpose

Expresses the MTase domain of NS5 of ZIKV

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

ZIKA virus NS5 methyltransferase domain

Tags

His6-sumo

Expression

Bacterial

Mutation

MTase domain aa 1-265

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_e19a2

Plasmid

#190884

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e19a2 BCR::ABL1 fusion cDNA
GUSB cDNA (GUSB Human)
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_e6a2

Plasmid

#190882

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts (e6a2)

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e6a2 cDNA
GUSB cDNA transcript (GUSB )
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_e8a2

Plasmid

#190883

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e8a2 BCR::ABL1 fusion cDNA
GUSB cDNA (GUSB Human)
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1

Plasmid

#194172

Purpose

Expresses lncRNA HIF1a-AS1 (spliced)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_deltaExon2

Plasmid

#194173

Purpose

Expresses lncRNA HIF1a-AS1 Exon1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Mutation

Exon2 was deleted

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_deltaExon1

Plasmid

#194174

Purpose

Expresses lncRNA HIF1a-AS1 Exon2

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Mutation

Exon1 was deleted

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_1200_unspliced

Plasmid

#194176

Purpose

Expresses first 1200 nt of lncRNA HIF1a-AS1 (unspliced)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-W80A-10xHis

Plasmid

#194179

Purpose

Expresses MPP8-W80A_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-W80A inside chromo domain

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta2-441-10xHis

Plasmid

#194181

Purpose

Expresses MPP8-delta2-441_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta2-441

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta442-860-10xHis

Plasmid

#194182

Purpose

Expresses MPP8-delta442-860_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta442-860

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta600-728-10xHis

Plasmid

#194183

Purpose

Expresses MPP8-delta600-728_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta600-728 (4xANK deletion)

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 C-V5-tag ORF7a

Plasmid

#179971

Purpose

Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 ORF7a (ORF7a Synthetic)

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP7 C-V5-tag

Plasmid

#179963

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 Nsp7 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.444Tmut

Plasmid

#190477

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Mutation

c.444G>T

Promoter

SV40

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 ORF6 C-V5-tag

Plasmid

#179970

Purpose

Mammalian expression vector for SARS-CoV-1 ORF6, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 ORF6 (ORF6 Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 E C-V5-tag

Plasmid

#179966

Purpose

Mammalian expression vector for SARS-CoV-1 E, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 E (E Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP16 C-V5-tag

Plasmid

#179965

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 Nsp16 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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