We narrowed to 9,745 results for: Tec;
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Plasmid#216520PurposeExpression of LegA7 ΔAnk290DepositorInsertLegA7 ΔAnk290
TagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 290-454Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7ΔNAnk264
Plasmid#216521PurposeExpression of LegA7 ΔNAnk264DepositorInsertLegA7 ΔNAnk264
TagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 264-361Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2/NTA/Leu-LegA7ΔNAnk290
Plasmid#216522PurposeExpression of LegA7 ΔNAnk290DepositorInsertLegA7 ΔNAnk290
TagsHIS-tagExpressionBacterial and YeastMutationLegA7 missing aa 290-361Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PRKCZ-C1 (N-PRKCZ-C1)
Plasmid#217760PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertProtein kinase C zeta (PRKCZ Human)
TagsEGFPExpressionMammalianMutationC1 domain (aa 123-193)PromoterCMVAvailable SinceJune 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
B-HeFSpCas9-A695Q
Plasmid#207368PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A695Q
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, K848A, Q926A, K1003A, R1060A, amino…PromoterCBhAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A926Q
Plasmid#207370PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A926Q
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, Q695A, K848A, K1003A, R1060A, amino…PromoterCBhAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A1060R
Plasmid#207372PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A1060R
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, amino …PromoterCBhAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A1003K
Plasmid#207371PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A1003K
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, Q695A, K848A, Q926A, R1060A, amino …PromoterCBhAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A848K
Plasmid#207369PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A848K
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…PromoterCBhAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A661R
Plasmid#207367PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A661R
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…PromoterCBhAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HypaR-SpCas9
Plasmid#126764PurposeExpression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.DepositorInsertB-Hypa2SpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationR661A; N692A; M694A; Q695A; H698A; amino acids 10…PromoterCBhAvailable SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
1K245-->R GLUT1-GFP
Plasmid#200095Purpose1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vectorDepositorInsertGLUT1 (SLC2A1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationK245RPromoterCMVAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-Pur-Trim69 isoform E (codon optimized)
Plasmid#199570PurposeStable and dox. inducible expression of Trim69 isoform E (codon optimized) after retroviral-mediated gene transferDepositorInsertTrim69 isoform E (codon optimized) (TRIM69 Human)
UseRetroviral; Allows for puromycin selectionTagsFlagMutationcodon optimizedAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-Pur-Trim69 isoform D (codon optimized)
Plasmid#199569PurposeStable and dox. inducible expression of Trim69 isoform D (codon optimized) after retroviral-mediated gene transferDepositorInsertTrim69 isoform D (codon optimized) (TRIM69 Human)
UseRetroviral; Allows for puromycin selectionTagsFlagMutationcodon optimizedAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-Pur-Trim69 isoform C (codon optimized)
Plasmid#199568PurposeStable and dox. inducible expression of Trim69 isoform C (codon optimized) after retroviral-mediated gene transferDepositorInsertTrim69 isoform C (codon optimized) (TRIM69 Human)
UseRetroviral; Allows for puromycin selectionTagsFlagMutationcodon optimizedAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-F41DW42D_AB
Plasmid#148372PurposeInsect Expression of Dm4E-T-F41DW42DDepositorInsertDm4E-T-F41DW42D (4E-T Fly)
ExpressionInsectMutation5 non silent mutation L127S, E493G, H628R, N659S,…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-mDia1-GBD (1-305 aa) in pEGFPN1
Plasmid#187280PurposeExpress EGFP-mDia1 GTPase-binding domainDepositorAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2326
Plasmid#169628PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2321
Plasmid#169626PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2329
Plasmid#169629PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
ExpressionMammalianPromoterPhCMV / PPGKAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only