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Pet22b-mCherry-TEAD4-IDR

Plasmid

#166442

Purpose

Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-IDR (TEAD4 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150-MIRΔ

Plasmid

#160995

Purpose

Express mouse p150 protein with MIR deletion mutation

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

MIR (HP1-interacting domain) deletion

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150-PIP1Δ

Plasmid

#160993

Purpose

Express mouse p150 protein with PIP1 deletion mutation

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

PIP1 (PCNA-interacting protein) motif deletion

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(2)-CRY2-mCherry

Plasmid

#160441

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pZA8

Plasmid

#158486

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Mutation

L17E, D481V, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MBC2-PuroIII

Plasmid

#153420

Purpose

MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisA-HOXC6-1

Plasmid

#110366

Purpose

Mammalian expression of HisA-tagged HOXC6-1

Depositor

Carlos Moreno

Article

Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

Insert

HOXC6 (HOXC6 Human)

Tags

HIS

Expression

Mammalian

Mutation

long isoform

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pETM30-ARC105

Plasmid

#133426

Purpose

Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

PCQAP (MED15 Human)

Expression

Bacterial

Mutation

Contains amino acids 5-78 fused to C-terminal of …

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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ETV3_pet28a

Plasmid

#131646

Purpose

Bacterial expression of ETS gene ETV3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV3 (ETV3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human APC-2,3 (1362–1540)

Plasmid

#132631

Purpose

expresses human APC-2,3 (1362–1540) in E. coli

Depositor

Wenqing Xu

Article

Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

Insert

APC-2,3 (1362–1540)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 WT

Plasmid

#126591

Purpose

Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant to GAGGAGAUAUUCAGACAUU

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXP2-hum

Plasmid

#129757

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

FOXP2 (LOC100411307 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

T301N/N323S

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHybr_r2a>mG3-srt-his

Plasmid

#124805

Purpose

HDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.

Depositor

Ferenc Scheeren, Martijn Verdoes

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Murine IgG3

Use

CRISPR; Hdr template

Tags

Histag (HHHHHH) and Sortag (LPETGG)

Expression

Bacterial and Mammalian

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Pconst-rfp + PmutS-yfp

Plasmid

#121444

Purpose

plasmid reporting the expression from constitutive and mutS promoters

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-yfp
Pconst-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

P-const and P-mutS

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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ERECTA C6_pECIA14

Plasmid

#114927

Purpose

Prey vector ERECTA C6_pECIA14 should be used with bait vector ERECTA C6_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G26330 (ER Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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PacrAB-rfp + Pconst-yfp

Plasmid

#121445

Purpose

plasmid reporting the expression from acrAB and constitutive promoters

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

Pconst-yfp
PacrAB-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

PacrAB and Pconst

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/Flag-tGFP-Cryz

Plasmid

#110378

Purpose

Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system

Depositor

Sandra Martha Gomes Dias

Insert

crystallin zeta (CRYZ Human)

Tags

FLAG and tGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMTgR2

Plasmid

#107286

Purpose

Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

Depositor

Bohdan Schneider

Article

Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

Insert

Interferon gamma receptor 2 (IFNGR2 Human)

Tags

CATCATCACCATCACCATGA

Expression

Insect

Promoter

Metallothionein promoter

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/V5-Foxa1

Plasmid

#105507

Purpose

MSCV-driven retroviral Foxa1 expression (V5-tagged)

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (Foxa1 Mouse)

Use

Retroviral

Tags

V5

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pJExpress414_GmPOPB-H698N

Plasmid

#92242

Purpose

Express H698N mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698N mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Asparagine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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